Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Aug;73(8):2716–2719. doi: 10.1073/pnas.73.8.2716

Direct spectroscopic observation of inner and outer hydrocarbon chains of lipid bilayer vesicles.

K J Longmuir, F W Dahlquist
PMCID: PMC430719  PMID: 1066684

Abstract

Distearoylphosphatidylcholines labeled with gem-difluoro groups at selected positions of the fatty acid chains have been prepared. These lipids were incorporated into sonicated vesicles and were examined by fluorine nuclear magnetic resonance spectroscopy. All spectra are characterized by two well-separated fluorine resonances. Paramagnetic ions added to the exterior solution of vesicles of fluorinated lipid broaden the upfield resonances, whereas ions trapped in the vesicles interior broaden the downfield resonances. This demonstrates that the fluorine chemical shift of these compounds is sensitive to differences between the inner and outer monolayers of sonicated vesicles.

Full text

PDF
2716

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boss W. F., Kelley C. J., Landsberger F. R. A novel synthesis of spin label derivatives of phosphatidylcholine. Anal Biochem. 1975 Mar;64(1):289–292. doi: 10.1016/0003-2697(75)90432-7. [DOI] [PubMed] [Google Scholar]
  2. Cubero Robles E., van den Berg D. Synthesis of lecithins by acylation of O-(sn-glycero-3-phosphoryl) choline with fatty acid anhydrides. Biochim Biophys Acta. 1969 Dec 17;187(4):520–526. doi: 10.1016/0005-2760(69)90049-6. [DOI] [PubMed] [Google Scholar]
  3. Huang C. H., Sipe J. P., Chow S. T., Martin R. B. Differential interaction of cholesterol with phosphatidylcholine on the inner and outer surfaces of lipid bilayer vesicles. Proc Natl Acad Sci U S A. 1974 Feb;71(2):359–362. doi: 10.1073/pnas.71.2.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Seelig A., Seelig J. The dynamic structure of fatty acyl chains in a phospholipid bilayer measured by deuterium magnetic resonance. Biochemistry. 1974 Nov 5;13(23):4839–4845. doi: 10.1021/bi00720a024. [DOI] [PubMed] [Google Scholar]
  5. Sheetz M. P., Chan S. I. Effect of sonication on the structure of lecithin bilayers. Biochemistry. 1972 Nov 21;11(24):4573–4581. doi: 10.1021/bi00774a024. [DOI] [PubMed] [Google Scholar]
  6. Stockton G. W., Polnaszek C. F., Tulloch A. P., Hasan F., Smith I. C. Molecular motion and order in single-bilayer vesicles and multilamellar dispersions of egg lecithin and lecithin-cholesterol mixtures. A deuterium nuclear magnetic resonance study of specifically labeled lipids. Biochemistry. 1976 Mar 9;15(5):954–966. doi: 10.1021/bi00650a003. [DOI] [PubMed] [Google Scholar]
  7. Waggoner A. S., Kingzett T. J., Rottschaefer S., Griffith O. H., Keith A. D. A spin-labeled lipid for probing biological membranes. Chem Phys Lipids. 1969 Sep;3(3):245–253. doi: 10.1016/0009-3084(69)90016-4. [DOI] [PubMed] [Google Scholar]
  8. Wells M. A., Hanahan D. J. Studies on phospholipase A. I. Isolation and characterization of two enzymes from Crotalus adamanteus venom. Biochemistry. 1969 Jan;8(1):414–424. doi: 10.1021/bi00829a057. [DOI] [PubMed] [Google Scholar]
  9. de Kruijff B., Cullis P. R., Radda G. K. Differential scanning calorimetry and 31P NMR studies on sonicated and unsonicated phosphatidylcholine liposomes. Biochim Biophys Acta. 1975 Sep 16;406(1):6–20. doi: 10.1016/0005-2736(75)90038-3. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES