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. 1976 Aug;73(8):2798–2802. doi: 10.1073/pnas.73.8.2798

Organization and energy-dependent growth of microtubules in cells.

F R Frankel
PMCID: PMC430751  PMID: 785472

Abstract

The organization and growth of microtubules in cultured mouse macrophages and fibroblasts were examined by indirect immunofluorescence microscopy with antibodies to microtubule protein. In macrophages, microtubules converged at a samll region at the cytocenter. During depolymerization, and repolymerization, this region acted as a microtubule organizing center. Microtubule growth was energy-dependent, but unaffected by dibutyryl-adenosine 3':5'-cyclic monophosphate, cholera toxin, or dibutyryl-guanosine 3':5'-cyclic monophosphate. Fibroblasts, which did not show such a simple microtubule organization as macrophages, contained mainly one or two, but occasionally as many as four, organizing centers during repolymerization. These microtubule organizing centers often appeared as fluorescent rings with a dark center.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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