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. 1976 Aug;73(8):2818–2822. doi: 10.1073/pnas.73.8.2818

Decreased fidelity of DNA polymerase activity isolated from aging human fibroblasts.

S Linn, M Kairis, R Holliday
PMCID: PMC430760  PMID: 1066693

Abstract

DNA polymerase (deoxynucleosidetriphosphate: DNA nucleotidyltransferase, EC 2.7.7.7 or DNA nucleotidyltransferase) activity, isolated from late and early passage cells of the diploid human fibroblast line, MRC-5, was compared. The level of activity dropped with increasing passage. In addition, when the fidelity of polymerization was monitored with four synthetic templates under a variety of conditions, it was observed that the enzyme from late passage cells was more error-prone. The possible relation of these observations to "senescence" of the fibroblasts is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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