Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Aug;73(8):2823–2827. doi: 10.1073/pnas.73.8.2823

Freeze-fracture study of mast cell secretion.

E Y Chi, D Lagunoff, J K Koehler
PMCID: PMC430762  PMID: 183213

Abstract

Within seconds after exposure of rat peritoneal mast cells to polymyxin B, bulges appear on the surface of the cells. Freeze-fracture electron microscopy reveals that each bulge overlies a mast cell granule. In contrast to the even distribution of intramembranous particles in the plasma membrane of unstimulated cells, the intramembranous particles in the stimulated cells are unevenly distributed in the membrane of the bulges with large patches of membrane lacking intramembranous particles. The membranes over the most prominent bulges are entirely free of intramembranous particles, and in some instances there is an increased concentration of intramembranous particles at the margins of the bulges. Perigranule membranes exhibit the same changes in distribution of intramembranous particles. Electron microscopy of thin sections of rapidly fixed, stimulated mast cells shows a peculiar structure of the membrane overlying some bulges; instead of the pentalaminar membranes previously demonstrated, the membrane at these sites of presumptive fusion of perigranule and plasma membrane assumes the form of a single dense lamina with a fine fuzzy coating on either side. It seems possible that membrane fusion and subsequent pore formation proceed in the stimulated mast cell through a stage of flight of intramembranous particles and molecular rearrangement of the other membrane components.

Full text

PDF
2823

Images in this article

2824Image
on p.2824
2825Image
on p.2825
2826Image
on p.2826

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahkong Q. F., Fisher D., Tampion W., Lucy J. A. Mechanisms of cell fusion. Nature. 1975 Jan 17;253(5488):194–195. doi: 10.1038/253194a0. [DOI] [PubMed] [Google Scholar]
  2. Anderson P., Slorach S. A., Uvnäs B. Sequential exocytosis of storage granules during antigen-induced histamine release from sensitized rat mast cells in vitro. An electron microscopic study. Acta Physiol Scand. 1973 Jul;88(3):359–372. doi: 10.1111/j.1748-1716.1973.tb05465.x. [DOI] [PubMed] [Google Scholar]
  3. Bloom G. D., Haegermark O. A study on morphological changes and histamine release induced by compound 48/80 in rat peritoneal mast cells. Exp Cell Res. 1965 Dec;40(3):637–654. doi: 10.1016/0014-4827(65)90241-7. [DOI] [PubMed] [Google Scholar]
  4. Branton D., Bullivant S., Gilula N. B., Karnovsky M. J., Moor H., Mühlethaler K., Northcote D. H., Packer L., Satir B., Satir P. Freeze-etching nomenclature. Science. 1975 Oct 3;190(4209):54–56. doi: 10.1126/science.1166299. [DOI] [PubMed] [Google Scholar]
  5. Chi E. Y., Lagunoff D., Koehler J. K. Electron microscopy of freeze-fractured rat peritoneal mast cells. J Ultrastruct Res. 1975 Apr;51(1):46–54. doi: 10.1016/s0022-5320(75)80007-4. [DOI] [PubMed] [Google Scholar]
  6. Daikoku S., Takahashi T., Kojimoto H., Watanabe Y. G. Secretory surface phenomena in freeze-etched preparations of the adenohypophysial cells and neurosecretory fibers. Z Zellforsch Mikrosk Anat. 1973;136(2):207–214. doi: 10.1007/BF00307441. [DOI] [PubMed] [Google Scholar]
  7. Horsfield G. I. The effect of compound 48/80 on the rat mast cell. J Pathol Bacteriol. 1965 Oct;90(2):599–605. doi: 10.1002/path.1700900228. [DOI] [PubMed] [Google Scholar]
  8. Kagayama M., Douglas W. W. Electron microscope evidence of calcium-induced exocytosis in mast cells treated with 48-80 or the ionophores A-23187 and X-537A. J Cell Biol. 1974 Aug;62(2):519–526. doi: 10.1083/jcb.62.2.519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Krüger G., Bloom D., Diamant B. Structural aspects of histamine release in rat peritoneal mast cells. Effects of adenosine 5'-triphosphate and role of calcium. Int Arch Allergy Appl Immunol. 1974;47(1):1–13. doi: 10.1159/000231196. [DOI] [PubMed] [Google Scholar]
  10. Krüger P. G., Bloom G. D. Structural features of histamine release in rat peritoneal mast cells. A study with toluidine blue. Int Arch Allergy Appl Immunol. 1974;46(5):740–752. doi: 10.1159/000231174. [DOI] [PubMed] [Google Scholar]
  11. LAGUNOFF D., PHILLIPS M. T., ISERI O. A., BENDITT E. P. ISOLATION AND PRELIMINARY CHARACTERIZATION OF RAT MAST CELL GRANULES. Lab Invest. 1964 Nov;13:1331–1344. [PubMed] [Google Scholar]
  12. Lagunoff D., Chi E. Y., Wan H. Effects of chymotrypsin and trypsin on rat peritoneal mast cells. Biochem Pharmacol. 1975 Sep 1;24(17):1573–1578. doi: 10.1016/0006-2952(75)90081-7. [DOI] [PubMed] [Google Scholar]
  13. Lagunoff D. Contributions of electron microscopy to the study of mast cells. J Invest Dermatol. 1972 May;58(5):296–311. doi: 10.1111/1523-1747.ep12540314. [DOI] [PubMed] [Google Scholar]
  14. Lagunoff D. Membrane fusion during mast cell secretion. J Cell Biol. 1973 Apr;57(1):252–259. doi: 10.1083/jcb.57.1.252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lagunoff D. The mechanism of histamine release from mast cells. Biochem Pharmacol. 1972 Jul 15;21(14):1889–1896. doi: 10.1016/0006-2952(72)90001-9. [DOI] [PubMed] [Google Scholar]
  16. Lagunoff D., Wan H. Temperature dependence of mast cell histamine secretion. J Cell Biol. 1974 Jun;61(3):809–811. doi: 10.1083/jcb.61.3.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MOTA I., BERALDO W. T., FERRI A. G., JUNQUEIRA L. C. Intracellular distribution of histamine. Nature. 1954 Oct 9;174(4432):698–698. doi: 10.1038/174698a0. [DOI] [PubMed] [Google Scholar]
  18. McIntyre J. A., Gilula N. B., Karnovsky M. J. Cryoprotectant-induced redistribution of intramembranous particles in mouse lymphocytes. J Cell Biol. 1974 Jan;60(1):192–203. doi: 10.1083/jcb.60.1.192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Orci L., Amherdt M., Malaisse-Lagae F., Rouiller C., Renold A. E. Insulin release by emiocytosis: demonstration with freeze-etching technique. Science. 1973 Jan 5;179(4068):82–84. doi: 10.1126/science.179.4068.82. [DOI] [PubMed] [Google Scholar]
  20. Orci L., Perrelet A. Membrane-associated particles: increase at sites of pinocytosis demonstrated by freeze-etching. Science. 1973 Aug 31;181(4102):868–869. doi: 10.1126/science.181.4102.868. [DOI] [PubMed] [Google Scholar]
  21. Pfenninger K., Akert K., Moor H., Sandri C. The fine structure of freeze-fractured presynaptic membranes. J Neurocytol. 1972 Sep;1(2):129–149. doi: 10.1007/BF01099180. [DOI] [PubMed] [Google Scholar]
  22. Röhlich P., Anderson P., Uvnäs B. Electron microscope observations on compounds 48-80-induced degranulation in rat mast cells. Evidence for sequential exocytosis of storage granules. J Cell Biol. 1971 Nov;51(21):465–483. doi: 10.1083/jcb.51.2.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Satir B., Schooley C., Satir P. Membrane fusion in a model system. Mucocyst secretion in Tetrahymena. J Cell Biol. 1973 Jan;56(1):153–176. doi: 10.1083/jcb.56.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Singleton E. M., Clark S. L., Jr The response of mast cells to compound 48/80 studied with the electron microscope. Lab Invest. 1965 Oct;14(10):1744–1763. [PubMed] [Google Scholar]
  25. Smith U., Smith D. S., Winkler H., Ryan J. W. Exocytosis in the adrenal medulla demonstrated by freeze-etching. Science. 1973 Jan 5;179(4068):79–82. doi: 10.1126/science.179.4068.79. [DOI] [PubMed] [Google Scholar]
  26. Uvnäs B., Aborg C. H., Bergendorff A. Storage of histamine in mast cells. Evidence for an ionic binding of histamine to protein carboxyls in the granule heparin-protein complex. Acta Physiol Scand Suppl. 1970;336:1–26. [PubMed] [Google Scholar]
  27. Wunderlich F., Wallach D. F., Speth V., Fischer H. Differential effects of temperature on the nuclear and plasma membranes of lymphoid cells. A study by freeze-etch electron microscopy. Biochim Biophys Acta. 1974 Nov 27;373(1):34–43. doi: 10.1016/0005-2736(74)90102-3. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES