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. 1977 Apr;74(4):1610–1613. doi: 10.1073/pnas.74.4.1610

Synthesis and activation of mitotic Ca2+-adenosinetriphosphatase during the cell cycle of mouse mastocytoma cells.

C Petzelt, D Auel
PMCID: PMC430840  PMID: 140377

Abstract

The activity of a Ca2+-adenosinetriphosphatase (ATP phosphohydrolase, EC 3.6.1.3) increases during the mitotic phase of synchronized mouse mastocytoma P-815X2 cells. The enzyme is synchronized mouse mastocytoma P-815X2 cells. The enzyme is synthesized mainly during a distinct period of the interphase and is activated at mitosis. It is thought to regulate the formation of the mitotic apparatus by controlling the concentration of Ca2+ ions at the site of formation of the mitotic spindle.

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Selected References

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  1. BIBRING T., COUSINEAU G. H. PERCENTAGE INCORPORATION OF LEUCINE LABELLED WITH CARBON-14 INTO ISOLATED MITOTIC APPARATUS DURING EARLY DEVELOPMENT OF SEA URCHIN EGGS. Nature. 1964 Nov 21;204:805–807. doi: 10.1038/204805a0. [DOI] [PubMed] [Google Scholar]
  2. Byfield J. E., Scherbaum O. H. Temperature effect on protein synthesis in a heat-synchronized protozoan treated with actinomycin D. Science. 1967 Jun 16;156(3781):1504–1505. doi: 10.1126/science.156.3781.1504. [DOI] [PubMed] [Google Scholar]
  3. Eibl H., Lands W. E. A new, sensitive determination of phosphate. Anal Biochem. 1969 Jul;30(1):51–57. doi: 10.1016/0003-2697(69)90372-8. [DOI] [PubMed] [Google Scholar]
  4. GROSS P. R., COUSINEAU G. H. MACROMOLECULE SYNTHESIS AND THE INFLUENCE OF ACTINOMYCIN ON EARLY DEVELOPMENT. Exp Cell Res. 1964 Feb;33:368–395. doi: 10.1016/0014-4827(64)90002-3. [DOI] [PubMed] [Google Scholar]
  5. Harris P. The role of membranes in the ogranization of the mitotic apparatus. Exp Cell Res. 1975 Sep;94(2):409–425. doi: 10.1016/0014-4827(75)90507-8. [DOI] [PubMed] [Google Scholar]
  6. Hayashi M., Matsumura F. Calcium binding to bovine tubulin. FEBS Lett. 1975 Oct 15;58(1):222–225. doi: 10.1016/0014-5793(75)80264-x. [DOI] [PubMed] [Google Scholar]
  7. JACOBSON B. S., SALMON R. J., LANSKY L. L. ANTIMITOTIC EFFECTS OF CHLORAMPHENICOL AND OTHER INHIBITORY AGENTS IN CHLAMYDOMONAS. Exp Cell Res. 1964 Oct;36:1–13. doi: 10.1016/0014-4827(64)90154-5. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Luykx P. Cellular mechanisms of chromosome distribution. Int Rev Cytol. 1970;(Suppl):1–173. [PubMed] [Google Scholar]
  11. Mazia D. Chromosome cycles turned on in unfertilized sea urchin eggs exposed to NH4OH. Proc Natl Acad Sci U S A. 1974 Mar;71(3):690–693. doi: 10.1073/pnas.71.3.690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mazia D. Microtubule research in perspective. Ann N Y Acad Sci. 1975 Jun 30;253:7–13. doi: 10.1111/j.1749-6632.1975.tb19188.x. [DOI] [PubMed] [Google Scholar]
  13. Mazia D., Petzelt C., Williams R. O., Meza I. A Ca-activated ATPase in the mitotic apparatus of the sea urchin egg (isolated by a new method). Exp Cell Res. 1972 Feb;70(2):325–332. doi: 10.1016/0014-4827(72)90143-7. [DOI] [PubMed] [Google Scholar]
  14. Nath J., Rebhun J. I. Effects of caffeine and other methylxanthines on the development and metabolism of sea urchin eggs. Involvement of NADP and glutathione. J Cell Biol. 1976 Mar;68(3):440–450. doi: 10.1083/jcb.68.3.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Parchman L. G., Stern H. The inhibition of protein synthesis in meiotic cells and its effect on chromosome behavior. Chromosoma. 1969;26(3):298–311. doi: 10.1007/BF00326524. [DOI] [PubMed] [Google Scholar]
  16. Petzelt C. Ca 2+ -activated APTase during the cell cycle of the sea urchin Strongylocentrotus purpuratus. Exp Cell Res. 1972 Feb;70(2):333–339. doi: 10.1016/0014-4827(72)90144-9. [DOI] [PubMed] [Google Scholar]
  17. Petzelt C. Further evidence that a Ca 2+ -activated ATPase is connected with the cell cycle. Exp Cell Res. 1972 Sep;74(1):156–162. doi: 10.1016/0014-4827(72)90491-0. [DOI] [PubMed] [Google Scholar]
  18. Petzelt C. NH3-treatment of unfertilized sea urchin eggs turns on the Ca2+-ATPase cycle. Exp Cell Res. 1976 Oct 1;102(1):200–204. doi: 10.1016/0014-4827(76)90315-3. [DOI] [PubMed] [Google Scholar]
  19. Petzelt C. UV-irradiation of sea urchin eggs changes the course of the Ca2+-activated ATPase activity. Exp Cell Res. 1974 Jun;86(2):404–408. doi: 10.1016/0014-4827(74)90731-9. [DOI] [PubMed] [Google Scholar]
  20. Petzelt C., von Ledebur-Villiger M. Ca2+-stimulated ATPase during the early development of parthenogenetically activated eggs of the sea urchin Paracentrotus lividus. Exp Cell Res. 1973 Sep;81(1):87–94. doi: 10.1016/0014-4827(73)90114-6. [DOI] [PubMed] [Google Scholar]
  21. RUSTAD R. C. Changes in the sensitivity to ultraviolet-induced mitotic delay during the cell division cycle of the sea urchin egg. Exp Cell Res. 1960 Dec;21:596–602. doi: 10.1016/0014-4827(60)90294-9. [DOI] [PubMed] [Google Scholar]
  22. Raff R. A., Brandis J. W., Green L. H., Kaumeyer J. F., Raff E. C. Microtubule protein pools in early development. Ann N Y Acad Sci. 1975 Jun 30;253:304–317. doi: 10.1111/j.1749-6632.1975.tb19209.x. [DOI] [PubMed] [Google Scholar]
  23. Rosenfeld A. C., Zackroff R. V., Weisenberg R. C. Magnesium stimulation of calcium binding to tubulin and calcium induced depolymerization of microtubules. FEBS Lett. 1976 Jun 1;65(2):144–147. doi: 10.1016/0014-5793(76)80466-8. [DOI] [PubMed] [Google Scholar]
  24. SCHINDLER R., DAY M., FISCHER G. A. Culture of neoplastic mast cells and their synthesis of 5-hydroxytryptamine and histamine in vitro. Cancer Res. 1959 Jan;19(1):47–51. [PubMed] [Google Scholar]
  25. Sachsenmaier W., Bohnert E., Clausnizer B., Nygaard O. F. Cycle dependent variation of X-ray effects on synchronous mitosis and thymidine kinase induction in Physarum polycephalum. FEBS Lett. 1970 Oct 5;10(3):185–189. doi: 10.1016/0014-5793(70)80449-5. [DOI] [PubMed] [Google Scholar]
  26. Schaer J. C., Schindler R. The requirement of mammalian cell cultures for serum proteins. Growth-promoting activity of pepsin-digested serum albumin in different media. Biochim Biophys Acta. 1967 Sep 19;147(1):154–161. doi: 10.1016/0005-2795(67)90098-0. [DOI] [PubMed] [Google Scholar]
  27. Schindler R., Schaer J. C. Preparation of synchronous cell cultures from early interphase cells obtained by sucrose gradient centrifugation. Methods Cell Biol. 1973;6:43–65. doi: 10.1016/s0091-679x(08)60047-3. [DOI] [PubMed] [Google Scholar]
  28. Timourian H., Jotz M. M., Clothier G. E. Intracellular distribution of calcium and phosphorus during the first cell division of the sea urchin egg. Exp Cell Res. 1974 Feb;83(2):380–386. doi: 10.1016/0014-4827(74)90352-8. [DOI] [PubMed] [Google Scholar]
  29. Weisenberg R. C. Microtubule formation in vitro in solutions containing low calcium concentrations. Science. 1972 Sep 22;177(4054):1104–1105. doi: 10.1126/science.177.4054.1104. [DOI] [PubMed] [Google Scholar]
  30. Wilt F. H., Sakai H., Mazia D. Old and new protein in the formation of the mitotic apparatus in cleaving sea urchin eggs. J Mol Biol. 1967 Jul 14;27(1):1–7. doi: 10.1016/0022-2836(67)90346-4. [DOI] [PubMed] [Google Scholar]
  31. Zeuthen E. Independent synchronization of DNA synthesis and of cell division in same culture of Tetrahymena cells. Demonstration that heat-shock induced division-synchrony is by differential extension of the G2 phase. Exp Cell Res. 1970 Aug;61(2):311–325. doi: 10.1016/0014-4827(70)90453-2. [DOI] [PubMed] [Google Scholar]

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