Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Sep;73(9):3025–3029. doi: 10.1073/pnas.73.9.3025

An RNA-dependent DNA polymerase, different from the known viral reverse transcriptases, in the chicken system.

G Bauer, P H Hofschneider
PMCID: PMC430915  PMID: 61587

Abstract

The properties of an RNA-dependent DNA polymerase (an RNA-dependent DNA nucleotidyltransferase), which occurs ubiquitously in the allantoic fluid of uninfected, leukosis-virus-free eggs, are described. It is shown that the enzyme can synthesize faithful transcripts from natural RNA (globin mRNA). By biochemical and immunological methods, the enzyme can be clearly distinguished from the reverse transcriptases of the known chicken RNA tumor viruses and therefore seems to be a member of a so far unknown class of chicken polymerases.

Full text

PDF
3025

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
  2. Bolden A., Fry M., Muller R., Citarella R., Weissbach A. The presence of a polyriboadenylic acid-dependent DNA polymerase in eukaryotic cells. Arch Biochem Biophys. 1972 Nov;153(1):26–33. doi: 10.1016/0003-9861(72)90416-x. [DOI] [PubMed] [Google Scholar]
  3. Brun G., Rougeon F., Lauber M., Chapeville F. Purification and properties of DNA polymerases from chick embryo. Eur J Biochem. 1974 Jan 16;41(2):241–251. doi: 10.1111/j.1432-1033.1974.tb03265.x. [DOI] [PubMed] [Google Scholar]
  4. Kang C. Y., Temin H. M. Endogenous RNA-directed DNA polymerase activity in uninfected chicken embryos. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1550–1554. doi: 10.1073/pnas.69.6.1550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Marcus S. L., Modak M. J., Cavalieri L. F. Purification of avian myeloblastosis virus DNA polymerase by affinity chromatography on polycytidylate-agarose. J Virol. 1974 Oct;14(4):853–859. doi: 10.1128/jvi.14.4.853-859.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mizutani S., Temin H. M. Lack of serological relationship among DNA polymerases of avian leukosis-sarcoma viruses, reticuloendotheliosis viruses, and chicken cells. J Virol. 1973 Sep;12(3):440–448. doi: 10.1128/jvi.12.3.440-448.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mizutani S., Temin H. M. Specific serological relationships among partially purified DNA polymerases of avian leukosis-sarcoma viruses, reticuloendotheliosis viruses, and avian cells. J Virol. 1974 May;13(5):1020–1029. doi: 10.1128/jvi.13.5.1020-1029.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moelling K., Gelderblom H., Pauli G., Friis R. A comparative study of the avian reticuloendotheliosis virus: relationship to murine leukemia virus and viruses of the avian sarcoma-leukosis complex. Virology. 1975 Jun;65(2):546–557. doi: 10.1016/0042-6822(75)90059-8. [DOI] [PubMed] [Google Scholar]
  9. Nowinski R. C., Watson K. F., Yaniv A., Spiegelman S. Serological analysis of the deoxyribonucleic acid polymerase of avian oncornaviruses. II. Comparison of avian deoxyribonucleic acid polymerases. J Virol. 1972 Nov;10(5):959–964. doi: 10.1128/jvi.10.5.959-964.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Panet A., Baltimore D., Hanafusa T. Quantitation of avian RNA tumor virus reverse transcriptase by radioimmunoassay. J Virol. 1975 Jul;16(1):146–152. doi: 10.1128/jvi.16.1.146-152.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ross J., Aviv H., Scolnick E., Leder P. In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer). Proc Natl Acad Sci U S A. 1972 Jan;69(1):264–268. doi: 10.1073/pnas.69.1.264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]
  13. Temin H. M. The cellular and molecular biology of RNA tumor viruses, especially avian leukosis-sarcoma viruses, and their relatives. Adv Cancer Res. 1974;19(0):47–104. doi: 10.1016/s0065-230x(08)60052-4. [DOI] [PubMed] [Google Scholar]
  14. Watson K. E., Nowinski R. C., Yaniv A., Spiegelman S. Serological analysis of the deoxyribonucleic acid polymerase of avian oncornaviruses. I. Preparation and characterization of monospecific antiserum with purified deoxyribonucleic acid polymerase. J Virol. 1972 Nov;10(5):951–958. doi: 10.1128/jvi.10.5.951-958.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weissbach A., Bolden A., Muller R., Hanafusa H., Hanafusa T. Deoxyribonucleic acid polymerase activities in normal and leukovirus-infected chicken embryo cells. J Virol. 1972 Sep;10(3):321–327. doi: 10.1128/jvi.10.3.321-327.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES