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. 1976 Sep;73(9):3220–3223. doi: 10.1073/pnas.73.9.3220

Mechanism of nuclear DNA replication in radicles of germinating cotton

Willard F Clay 1, Paul G Bartels 1, Frank R H Katterman 1
PMCID: PMC430984  PMID: 16592350

Abstract

The mechanism of nuclear DNA replication in radicles of germinating cotton (Gossypium barbadense) was investigated. Pulse-labeling with [3H]thymidine indicates that replication intermediates are of small molecular weight (4-10S) and behave as if single-stranded. Prolonged labeling indicates that intermediates are of discrete size, suggesting a mechanism of discontinuous replication. Electron microscopy of nuclear DNA demonstrates a complex architecture which may include “eye forms” and “forks” similar to those reported in prokaryotes and animal systems. The possible universality of DNA replication mechanisms is discussed.

Keywords: discontinuous DNA replication, electron microscopy, Okazaki fragments, eye forms

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Selected References

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