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Summary of the strategy adopted to obtain the M. persicae RyR cDNA sequence. Five overlapping fragments (F1a/b, F2, F3 and F4) were initially PCR amplified. Fragments F1a, F1b, F2 and F3 were sub-cloned into pJET1.2blunt vector (Thermo-Fermentas) and fragment F4 was sub-cloned into a TOPO®TA vector (Invitrogen) for sequencing.
