Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 May;74(5):1899–1902. doi: 10.1073/pnas.74.5.1899

Translation in vitro of rat liver messenger RNA coding for ligandin (glutathione S-transferase B).

V Daniel, G J Smith, G Litwack
PMCID: PMC431039  PMID: 266712

Abstract

Poly(A)-containing rat liver mRNA isolated from animals injected with phenobarbital and uninjected controls was translated efficiently in a wheat-germ system. The synthesis of ligandin (glutathione S-transferase B; glutathione transferase; RX-gluathione R-transferase, EC 2.5.1.18) was detected by immunoprecipitation with a highly purified monospecific ligandin antibody and analysis by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The extent of incorporation of [35S]methionine into ligandin in the translation system was similar for poly(A)-containing messages from un-infected animals and those treated with phenobarbital.

Full text

PDF
1899

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arias I. M., Doyle D., Schimke R. T. Studies on the synthesis and degradation of proteins of the endoplasmic reticulum of rat liver. J Biol Chem. 1969 Jun 25;244(12):3303–3315. [PubMed] [Google Scholar]
  2. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bannikov G. A., Guelstein V. I., Tchipysheva T. A. Distribution of basic azo-dye-binding protein in normal rat tissues and carcinogen-induced liver tumors. Int J Cancer. 1973 Mar 15;11(2):398–411. doi: 10.1002/ijc.2910110217. [DOI] [PubMed] [Google Scholar]
  4. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  5. Fleischner G., Robbins J., Arias I. M. Immunological studies of Y protein. A major cytoplasmic organic anion-binding protein in rat liver. J Clin Invest. 1972 Mar;51(3):677–684. doi: 10.1172/JCI106856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldberg A. L., St John A. C. Intracellular protein degradation in mammalian and bacterial cells: Part 2. Annu Rev Biochem. 1976;45:747–803. doi: 10.1146/annurev.bi.45.070176.003531. [DOI] [PubMed] [Google Scholar]
  7. Habig W. H., Pabst M. J., Fleischner G., Gatmaitan Z., Arias I. M., Jakoby W. B. The identity of glutathione S-transferase B with ligandin, a major binding protein of liver. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3879–3882. doi: 10.1073/pnas.71.10.3879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Habig W. H., Pabst M. J., Jakoby W. B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974 Nov 25;249(22):7130–7139. [PubMed] [Google Scholar]
  9. Ketterer B., Ross-Mansell P., Whitehead J. K. The isolation of carcinogen-binding protein from livers of rats given 4-dimethylaminoazobenzene. Biochem J. 1967 May;103(2):316–324. doi: 10.1042/bj1030316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Levi A. J., Gatmaitan Z., Arias I. M. Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anions. J Clin Invest. 1969 Nov;48(11):2156–2167. doi: 10.1172/JCI106182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Litwack G., Ketterer B., Arias I. M. Ligandin: a hepatic protein which binds steroids, bilirubin, carcinogens and a number of exogenous organic anions. Nature. 1971 Dec 24;234(5330):466–467. doi: 10.1038/234466a0. [DOI] [PubMed] [Google Scholar]
  12. Litwack G., Morey K. S. Cortisol metabolite binder. I. Identity with the dimethylaminoazobenzene binding protein of liver cytosol. Biochem Biophys Res Commun. 1970 Mar 27;38(6):1141–1148. doi: 10.1016/0006-291x(70)90358-x. [DOI] [PubMed] [Google Scholar]
  13. Morey K. S., Litwack G. Isolation and properties of cortisol metabolite binding proteins of rat liver cytosol. Biochemistry. 1969 Dec;8(12):4813–4821. doi: 10.1021/bi00840a024. [DOI] [PubMed] [Google Scholar]
  14. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  15. Reyes H., Levi A. J., Gatmaitan Z., Arias I. M. Organic anion-binding protein in rat liver: drug induction and its physiologic consequence. Proc Natl Acad Sci U S A. 1969 Sep;64(1):168–170. doi: 10.1073/pnas.64.1.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reyes H., Levi A. J., Gatmaitan Z., Arias I. M. Studies of Y and Z, two hepatic cytoplasmic organic anion-binding proteins: effect of drugs, chemicals, hormones, and cholestasis. J Clin Invest. 1971 Nov;50(11):2242–2252. doi: 10.1172/JCI106721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Taylor J. M., Schimke R. T. Synthesis of rat liver albumin in a rabbit reticulocyte cell-free protein-synthesizing system. J Biol Chem. 1973 Nov 25;248(22):7661–7668. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES