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. 1977 May;74(5):1965–1968. doi: 10.1073/pnas.74.5.1965

Role of DNA gyrase in phiX replicative-form replication in vitro.

K J Marians, J E Ikeda, S Schlagman, J Hurwitz
PMCID: PMC431053  PMID: 266716

Abstract

Preparations containing DNA gyrase activity Gellert, M., Mizuchi, K., O'Dea, M.H. & Nash, H.A. (1976) Proc. Natl. Acad. Sci. USA 73, 3872-3876] have been extensively purified from Escherichia coli. Such fractions, in the presence of ATP and Mg2+, catalyze supertwisting of relaxed circular double-stranded DNA replicative forms of a number of DNAs that results in the formation of superhelical replicative forms. Relaxed phiX174 replicative form (phiX RFIV) is not attacked by the A protein endonuclease coded for by the phiX DNA genome. After exposure to preparations of DNA gyrase, the relaxed phiX174 replicative form is converted to phiX RFI which can then be attacked by the phiX gene A protein and participate in replication of duplex phiX DNA.

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1965

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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