Abstract
When Drosophila melanogaster embryos were ligated at very early stages of nuclear multiplication (45-60 min after egg deposition) for the entire period of embryogenesis, egg fragments of variable sizes were able to continue development up to the hatching stage. These ligated embryos differentiated larval structures; anterior fragments formed larval head and posterior fragments formed larval abdominal structures. Ligation always prevented the differentiation of some of the intermediate larval bands whereas the terminal ones were always formed. Identical results were obtained when embryos (45-60 min after egg deposition) were temporarily ligated for only 5 min. However, puncturing with a fine glass needle immediately after temporary ligation through the ligated area could give rise to complete differentiation of all the bands. Since puncturing disrupted the barrier produced by ligation, we propose that interaction between anterior and posterior egg regions is necessary to allow complete development.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brown E. E., Schubiger G. Centrifugation of developing oocytes leading to embryonic defects. Dev Biol. 1977 Jan;55(1):170–178. doi: 10.1016/0012-1606(77)90328-1. [DOI] [PubMed] [Google Scholar]
- Gurdon J. B. Attmepts to analyse the biochemical basis of regional differences in animal eggs. Ciba Found Symp. 1975;0(29):223–239. doi: 10.1002/9780470720110.ch11. [DOI] [PubMed] [Google Scholar]
- Kauffman S. Control circuits for determination and transdetermination: interpreting positional information in a binary epigenetic code. Ciba Found Symp. 1975;0(29):201–221. doi: 10.1002/9780470720110.ch10. [DOI] [PubMed] [Google Scholar]
- Schubiger G. Adult differentiation from partial Drosophila embryos after egg ligation during stages of nuclear multiplication and cellular blastoderm. Dev Biol. 1976 Jun;50(2):476–488. doi: 10.1016/0012-1606(76)90166-4. [DOI] [PubMed] [Google Scholar]
- Wieschaus E., Gehring W. Clonal analysis of primordial disc cells in the early embryo of Drosophila melanogaster. Dev Biol. 1976 Jun;50(2):249–263. doi: 10.1016/0012-1606(76)90150-0. [DOI] [PubMed] [Google Scholar]