Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 May;74(5):2064–2068. doi: 10.1073/pnas.74.5.2064

High-fidelity transcription of 5S DNA injected into Xenopus oocytes.

D D Brown, J B Gurdon
PMCID: PMC431074  PMID: 266727

Abstract

Purified DNA, from Xenopus erythrocytes, containing genes for 5S ribosomal RNA (58 DNA) is transcribed faithfully when it is injected into the nucleus of a Xenopus oocyte. Full length 5S RNA of the correct sequence is transcribed from the injected DNA, and the newly synthesized transcripts hybridize predominantly with the coding strand of 5S DNA. The majority of transcripts hybridize with the gene region as opposed to the spacer region of the coding strand. Accurate transcription starts within the first 3 hr and continues for up to 4 days after injection of 5S DNA. Radioactive 5S RNA is detected in as short a labeling time as 1 hr.

Full text

PDF
2064

Images in this article

2065Image
on p.2065
2065Image
on p.2065
2066Image
on p.2066
2066Image
on p.2066

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  2. Brown D. D., Sugimoto K. 5 S DNAs of Xenopus laevis and Xenopus mulleri: evolution of a gene family. J Mol Biol. 1973 Aug 15;78(3):397–415. doi: 10.1016/0022-2836(73)90464-6. [DOI] [PubMed] [Google Scholar]
  3. Brown D. D., Sugimoto K. The structure and evolution of ribosomal and 5S DNAs in Xenopus laevis and Xenopus mulleri. Cold Spring Harb Symp Quant Biol. 1974;38:501–505. doi: 10.1101/sqb.1974.038.01.054. [DOI] [PubMed] [Google Scholar]
  4. Brown D. D., Weber C. S. Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA. J Mol Biol. 1968 Jun 28;34(3):661–680. doi: 10.1016/0022-2836(68)90188-5. [DOI] [PubMed] [Google Scholar]
  5. Brown D. D., Wensink P. C., Jordan E. Purification and some characteristics of 5S DNA from Xenopus laevis. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3175–3179. doi: 10.1073/pnas.68.12.3175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brown R. D., Brown D. D. The nucleotide sequence adjoining the 3' end of the genes coding for oocyte-type 5 S ribosomal RNA in Xenopus. J Mol Biol. 1976 Mar 25;102(1):1–14. doi: 10.1016/0022-2836(76)90070-x. [DOI] [PubMed] [Google Scholar]
  7. Brownlee G. G., Cartwright E. M., Brown D. D. Sequence studies of the 5 S DNA of Xenopus laevis. J Mol Biol. 1974 Nov 15;89(4):703–718. doi: 10.1016/0022-2836(74)90046-1. [DOI] [PubMed] [Google Scholar]
  8. Colman A. Synthesis of RNA in oocytes of Xenopus laevis during culture in vitro. J Embryol Exp Morphol. 1974 Oct;32(2):515–532. [PubMed] [Google Scholar]
  9. Commerford S. L. Iodination of nucleic acids in vitro. Biochemistry. 1971 May 25;10(11):1993–2000. doi: 10.1021/bi00787a005. [DOI] [PubMed] [Google Scholar]
  10. Denis H., Wegnez M., Willem R. Recherches biochimiques sur l'oogenèse. V. Comparaison entre le RNA 5 S somatique et le RNA 5 S des oocytes de Xenopus laevis. Biochimie. 1972;54(9):1189–1195. doi: 10.1016/s0300-9084(72)80023-3. [DOI] [PubMed] [Google Scholar]
  11. Dumont J. N. Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals. J Morphol. 1972 Feb;136(2):153–179. doi: 10.1002/jmor.1051360203. [DOI] [PubMed] [Google Scholar]
  12. Ford P. J., Brown R. D. Sequences of 5S ribosomal RNA from Xenopus mulleri and the evolution of 5S gene-coding sequences. Cell. 1976 Aug;8(4):485–493. doi: 10.1016/0092-8674(76)90216-6. [DOI] [PubMed] [Google Scholar]
  13. Gurdon J. B. Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal. J Embryol Exp Morphol. 1976 Dec;36(3):523–540. [PubMed] [Google Scholar]
  14. Honjo T., Reeder R. H. Preferential transcription of Xenopus laevis ribosomal RNA in interspecies hybrids between Xenopus laevis and Xenopus mulleri. J Mol Biol. 1973 Oct 25;80(2):217–228. doi: 10.1016/0022-2836(73)90168-x. [DOI] [PubMed] [Google Scholar]
  15. Mertz J. E., Gurdon J. B. Purified DNAs are transcribed after microinjection into Xenopus oocytes. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1502–1506. doi: 10.1073/pnas.74.4.1502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Parker C. S., Roeder R. G. Selective and accurate transcription of the Xenopus laevis 5S RNA genes in isolated chromatin by purified RNA polymerase III. Proc Natl Acad Sci U S A. 1977 Jan;74(1):44–48. doi: 10.1073/pnas.74.1.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Peacock A. C., Dingman C. W. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968 Feb;7(2):668–674. doi: 10.1021/bi00842a023. [DOI] [PubMed] [Google Scholar]
  18. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  19. Wegnez M., Monier R., Denis H. Sequence heterogeneity of 5 S RNA in Xenopus laevis. FEBS Lett. 1972 Sep 1;25(1):13–20. doi: 10.1016/0014-5793(72)80443-5. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES