Table I.

Secretion of serotonin, matrix-metalloproteinase-2 (MMP-2), beta-amyloid (Aβ).

	Controls	1 mM H2O2	10 mM H2O2
Serotonin	27 ± 3 (8) -	45.5 ± 29 (6) ns	30.5 ± 13 (3) ns
MMP-2	115 ± 24 (5) -	207 ± 99 (3) ns	131 ± 19 (3) ns
Aβ(1-40)	17.1 ± 0.6 (6) -	13.6 ± 2.4 (4) ns	19.9 ± 2.4 (6) ns
Aβ(1-42)	3.2 ± 1 (5) -	1.2 ± 0.4 (4) *	1.0 ± 0.4 (6) *

Rat platelets (1×10⁸) were incubated without (controls) or with 1 or 10mM hydrogen peroxide (H₂O₂) for 10 minutes (serotonin), 3 hours (MMP-2), or 24 hours (Aβ), then centrifuged and supernatant was evaluated by HPLC-EC (serotonin) or ELISA (MMP-2, Aβ). Values are given as mean ± SEM ng/mlx10 minutes (serotonin), pg/mlx3 hours (MMP-2), or pM/24 hours (Aβ). Statistical analysis was performed by one way analysis of variance with a subsequent Fisher LSD posthoc test. Values in parenthesis give the number of experiments.

^*p < 0.05;

ns, not significant.