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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Oct;73(10):3376–3380. doi: 10.1073/pnas.73.10.3376

Deficiency in plasma protein synthesis caused by x-ray-incuded lethal albino alleles in mouse.

R C Garland, J Satrustegui, S Gluecksohn-Waelsch, C F Cori
PMCID: PMC431117  PMID: 62358

Abstract

Plasma protein synthesis was studied in mice bearing x-ray induced lethal mutations at the albino locus. Newborn albino mutants showed a decrease in each of the three principal plasma proteins, albumin, alpha-fetoprotein, and transferrin, when compared with colored littermate controls. Incorporation of [14C]leucine into plasma proteins of the newborn albinos 30 min after injection was only 1/5 that of the controls, but incorporation into total liver protein was only slightly diminished. Incorporation of [14C]leucine into an albumin fraction obtained by immunoprecipitation from livers incubated in vitro in an amino acid mixture was also strongly diminished. Thus, the liver of 18-day-old albino fetuses incorporated into this fraction 1/3 and that of newborn albinos 1/8 as much as the controls, but in both cases the incorporation into total liver protein was only 25% less than in the respective controls. These results indicate that the rather severe structural abnormalities observed in the mutants in the endoplasmic reticulum and the Golgi apparatus are not associated with a general deficiency of hepatic protein synthesis. Instead the data from this and previous work show that the progressive deficiency from fetal life to birth involves certain specific proteins represented by several perinatally developing enzymes and by plasma proteins. It is suggested that the mutational effects observed in these mice are due to deletions involving regulatory rather than structural genes at or near the albino locus.

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Selected References

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  1. Dorling P. R., Quinn P. S., Judah J. D. Evidence for the coupling of biosynthesis and secretion of serum albumin in the rat. The effect of colchicine on albumin production. Biochem J. 1975 Nov;152(2):341–348. doi: 10.1042/bj1520341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ganoza M. C., Williams C. A. In vitro synthesis of different categories of specific protein by membrane-bound and free ribosomes. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1370–1376. doi: 10.1073/pnas.63.4.1370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Geller D. M., Judah J. D., Nicholls M. R. Intracellular distribution of serum albumin and its possible precursors in rat liver. Biochem J. 1972 May;127(5):865–874. doi: 10.1042/bj1270865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gluecksohn-Waelsch S., Schiffman M. B. Glutamine synthetase in newborn mice homozygous for lethal albino alleles. Dev Biol. 1975 Aug;45(2):369–371. doi: 10.1016/0012-1606(75)90075-5. [DOI] [PubMed] [Google Scholar]
  5. Gluecksohn-Waelsch S., Schiffman M. B., Thorndike J., Cori C. F. Complementation studies of lethal alleles in the mouse causing deficiencies of glucose-6-phosphatase, tyrosine aminotransferase, and serine dehydratase. Proc Natl Acad Sci U S A. 1974 Mar;71(3):825–829. doi: 10.1073/pnas.71.3.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gustine D. L., Zimmerman E. F. Developmental changes in microheterogeneity of foetal plasma glycoproteins of mice. Biochem J. 1973 Mar;132(3):541–551. doi: 10.1042/bj1320541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ikehara Y., Pitot H. C. Localization of polysome-bound albumin and serine dehydratase in rat liver cell fractions. J Cell Biol. 1973 Oct;59(1):28–44. doi: 10.1083/jcb.59.1.28. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kirsch R., Frith L., Black E., Hoffenberg R. Regulation of albumin synthesis and catabolism by alteration of dietary protein. Nature. 1968 Feb 10;217(5128):578–579. doi: 10.1038/217578a0. [DOI] [PubMed] [Google Scholar]
  9. Koga K., Tamaoki T. Developmental changes in the synthesis of alpha-fetoprotein and albumin in the mouse liver. Cell-free synthesis by membrane-bound polyribosomes. Biochemistry. 1974 Jul 16;13(15):3024–3028. doi: 10.1021/bi00712a004. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Lin C., Chang J. Electron microscopy of albumin synthesis. Science. 1975 Oct 31;190(4213):465–467. doi: 10.1126/science.170682. [DOI] [PubMed] [Google Scholar]
  12. Miller O. J., Miller D. A. The mouse chromosome map. Fed Proc. 1975 Dec;34(13):2218–2221. [PubMed] [Google Scholar]
  13. Morgan E. H., Peters T., Jr The biosynthesis of rat serum albumin. V. Effect of protein depletion and refeeding on albumin and transferrin synthesis. J Biol Chem. 1971 Jun 10;246(11):3500–3507. [PubMed] [Google Scholar]
  14. Nichols E. A., Ruddle F. H., Petras M. L. Linkage of the locus for serum albumin in the house mouse, Mus musculus. Biochem Genet. 1975 Oct;13(9-10):551–555. doi: 10.1007/BF00484914. [DOI] [PubMed] [Google Scholar]
  15. Palade G. Intracellular aspects of the process of protein synthesis. Science. 1975 Aug 1;189(4200):347–358. doi: 10.1126/science.1096303. [DOI] [PubMed] [Google Scholar]
  16. Peters T., Jr, Peters J. C. The biosynthesis of rat serum albumin. VI. Intracellular transport of albumin and rates of albumin and liver protein synthesis in vivo under various physiological conditions. J Biol Chem. 1972 Jun 25;247(12):3858–3863. [PubMed] [Google Scholar]
  17. Redman C. M. Biosynthesis of serum proteins and ferritin by free and attached ribosomes of rat liver. J Biol Chem. 1969 Aug 25;244(16):4308–4315. [PubMed] [Google Scholar]
  18. Rothman S. S. Protein transport by the pancreas. Science. 1975 Nov 21;190(4216):747–753. doi: 10.1126/science.1105785. [DOI] [PubMed] [Google Scholar]
  19. Schreiber G., Urban J., Zähringer J., Reutter W., Frosch U. The secretion of serum protein and the synthesis of albumin and total protein in regenerating rat liver. J Biol Chem. 1971 Jul 25;246(14):4531–4538. [PubMed] [Google Scholar]
  20. Trigg M. J., Gluecksohn-Waelsch S. Ultrastructural basis of biochemical effects in a series of lethal alleles in the mouse. Neonatal and developmental studies. J Cell Biol. 1973 Sep;58(3):549–563. doi: 10.1083/jcb.58.3.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yeoh G. C. Albumin and transferrin synthesis in the liver of rats in the immediate postnatal period. Biochim Biophys Acta. 1973 Dec 21;331(3):421–429. doi: 10.1016/0005-2787(73)90028-2. [DOI] [PubMed] [Google Scholar]
  22. Yeoh G. C., Morgan E. H. Albumin and transferrin synthesis during development in the rat. Biochem J. 1974 Nov;144(2):215–224. doi: 10.1042/bj1440215. [DOI] [PMC free article] [PubMed] [Google Scholar]

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