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. 1976 Oct;73(10):3438–3442. doi: 10.1073/pnas.73.10.3438

Novel structure at 5'-ends of nascent DNA chains.

D W Siegmann, R Werner
PMCID: PMC431130  PMID: 185611

Abstract

Because of their association with protein short nascent DNA chains in Escherichia coli can be separated from other cellular DNA by chromatography on hydroxylapatite. Protein-free DNA chains of less than 500 nucleotides in length are resistant to degradation from the 5'-end by alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum); EC 3.1.3.1] and spleen phosphodiesterase (oligonucleate 3'-nucleotidohydrolase; EC 3.1.4.18). In contrast, DNA chains containing more than 500 nucleotides are degradable. From these results we conclude that short nascent DNA chains are structurally modified at their 5'-ends. The nature of this structure and its possible functions are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brewin N. [Catalytic role for RNA in DNA replication]. Nat New Biol. 1972 Mar 29;236(65):101–101. doi: 10.1038/newbio236101a0. [DOI] [PubMed] [Google Scholar]
  2. Danna K. J., Sack G. H., Jr, Nathans D. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome. J Mol Biol. 1973 Aug 5;78(2):363–376. doi: 10.1016/0022-2836(73)90122-8. [DOI] [PubMed] [Google Scholar]
  3. Diaz A. T., Werner R. Mechanism of DNA chain growth. J Mol Biol. 1975 Jun 15;95(1):63–70. doi: 10.1016/0022-2836(75)90335-6. [DOI] [PubMed] [Google Scholar]
  4. Diaz A. T., Wiener D., Werner R. Synthesis of small polynucleotide chains in thymine-depleted bacteria. J Mol Biol. 1975 Jun 15;95(1):45–61. doi: 10.1016/0022-2836(75)90334-4. [DOI] [PubMed] [Google Scholar]
  5. Furuichi Y., Morgan M., Muthukrishnan S., Shatkin A. J. Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-. Proc Natl Acad Sci U S A. 1975 Jan;72(1):362–366. doi: 10.1073/pnas.72.1.362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hirose S., Okazaki R., Tamanoi F. Mechanism of DNA chain growth. XI. Structure of RNA-linked DNA fragments of Escherichia coli. J Mol Biol. 1973 Jul 15;77(4):501–517. doi: 10.1016/0022-2836(73)90219-2. [DOI] [PubMed] [Google Scholar]
  7. Jacobson M. K., Lark K. G. DNA replication in Escherichia coli: evidence for two classes of small deoxyribonucleotide chains. J Mol Biol. 1973 Feb 5;73(4):371–396. doi: 10.1016/0022-2836(73)90088-0. [DOI] [PubMed] [Google Scholar]
  8. Konrad E. B., Lehman I. R. Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2150–2154. doi: 10.1073/pnas.72.6.2150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kurosawa Y., Ogawa T., Hirose S., Okazaki T., Okazaki R. Mechanism of DNA chain growth. XV. RNA-linked nascent DNA pieces in Escherichia coli strains assayed with spleen exonuclease. J Mol Biol. 1975 Aug 25;96(4):653–664. doi: 10.1016/0022-2836(75)90144-8. [DOI] [PubMed] [Google Scholar]
  10. Lark K. G., Wechsler J. A. DNA replication in dnaB mutants of Escherichia coli: gene product interaction and synthesis of 4 S pieces. J Mol Biol. 1975 Feb 15;92(1):145–163. doi: 10.1016/0022-2836(75)90095-9. [DOI] [PubMed] [Google Scholar]
  11. Oishi M. Studies of DNA replication in vivo. I. Isolation of the first intermediate of DNA replication in bacteria as single-stranded DNA. Proc Natl Acad Sci U S A. 1968 May;60(1):329–336. doi: 10.1073/pnas.60.1.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Okazaki T., Okazaki R. Mechanism of DNA chain growth. IV. Direction of synthesis of T4 short DNA chains as revealed by exonucleolytic degradation. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1242–1248. doi: 10.1073/pnas.64.4.1242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Olivera B. M., Hall Z. W., Anraku Y., Chien J. R., Lehman I. R. On the mechanism of the polynucleotide joining reaction. Cold Spring Harb Symp Quant Biol. 1968;33:27–34. doi: 10.1101/sqb.1968.033.01.008. [DOI] [PubMed] [Google Scholar]
  14. Wang H. F., Sternglanz R. Thymine-labelled deoxyoligonucleotide involved in DNA chain growth in Bacillus subtilis. Nature. 1974 Mar 8;248(5444):147–150. doi: 10.1038/248147a0. [DOI] [PubMed] [Google Scholar]
  15. Werner R. Mechanism of DNA replication. Nature. 1971 Apr 30;230(5296):570–572. doi: 10.1038/230570a0. [DOI] [PubMed] [Google Scholar]
  16. Werner R. Nature of DNA precursors. Nat New Biol. 1971 Sep 22;233(38):99–103. doi: 10.1038/newbio233099a0. [DOI] [PubMed] [Google Scholar]

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