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. 1976 Oct;73(10):3539–3543. doi: 10.1073/pnas.73.10.3539

Partial purification and characterization of the mRNA for rat preproinsulin.

J R Duguid, D F Steiner, W L Chick
PMCID: PMC431152  PMID: 185618

Abstract

Electrophoretically homogeneous messenger RNA for rat preproinsulin has been prepared from an islet cell tumor by the use of oligo(dT(-cellulose chromatography and sucrose density gradient centrifugation. The molecular weight of the mRNA is about 210,000, as determined by polyacrylamide gel electrophoresis in formamide, and its sedimentation coefficient is 9.3 S in sucrose gradients containing 0.2 M NaCl. These results indicate that the synthesis of preproinsulin is directed by a monocistronic mRNA 600 nucleotides in length and requires approximately 55% of the molecule's coding capacity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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