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. 1976 Oct;73(10):3554–3557. doi: 10.1073/pnas.73.10.3554

Temperature dependence of the four ionic processes of spike electrogenesis in eel electroplaques.

F Ruiz-Manresa, H Grundfest
PMCID: PMC431155  PMID: 1068467

Abstract

Spike electrogenesis of eel electroplaques involves four ionic processes which are controlled by the membrane potential. A threshold depolarization causes normally closed Na permselective channels to open (Na-activation) and normally open K channels to close (K-inactivation). The Na channels then close (Na-inactivation), and as the spike is terminated, the K channels reopen (K-reactivation). The temperature dependence of these four processes has been examined in the present work. Opening of the Na channels and closure and reopening of the K-channels are either effectively instantaneous or are relatively independent of temperature in the range of at least 5 degrees to 22 degrees. Closure of the Na-channels has a Q10 (increase in rate of reaction for each 10 degrees increase in temperature) of about 9, and activation energy (Ea) of this reaction is about 31.5 kcal/mole (132 kJ/mole).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CORABOEUF E., WEIDMANN S. Temperature effects on the electrical activity of Purkinje fibres. Helv Physiol Pharmacol Acta. 1954;12(1):32–41. [PubMed] [Google Scholar]
  2. FRANKENHAEUSER B., MOORE L. E. THE EFFECT OF TEMPERATURE ON THE SODIUM AND POTASSIUM PERMEABILITY CHANGES IN MYELINATED NERVE FIBRES OF XENOPUS LAEVIS. J Physiol. 1963 Nov;169:431–437. doi: 10.1113/jphysiol.1963.sp007269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GRUNDFEST H. Ionic mechanisms in electrogenesis. Ann N Y Acad Sci. 1961 Sep 6;94:405–457. doi: 10.1111/j.1749-6632.1961.tb35554.x. [DOI] [PubMed] [Google Scholar]
  4. GRUNDFEST H. The mechanisms of discharge of the electric organs in relation to general and comparative electrophysiology. Prog Biophys Biophys Chem. 1957;7:1–85. [PubMed] [Google Scholar]
  5. Goldman L. Quantitative description of the sodium conductance of the giant axon of Myxicola in terms of a generalized second-order variable. Biophys J. 1975 Feb;15(2 Pt 1):119–136. doi: 10.1016/s0006-3495(75)85796-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HODGKIN A. L., HUXLEY A. F. A quantitative description of membrane current and its application to conduction and excitation in nerve. J Physiol. 1952 Aug;117(4):500–544. doi: 10.1113/jphysiol.1952.sp004764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HUXLEY A. F. Ion movements during nerve activity. Ann N Y Acad Sci. 1959 Aug 28;81:221–246. doi: 10.1111/j.1749-6632.1959.tb49311.x. [DOI] [PubMed] [Google Scholar]
  8. KEYNES R. D., MARTINS-FERREIRA H. Membrane potentials in the electroplates of the electric eel. J Physiol. 1953 Feb 27;119(2-3):315–351. doi: 10.1113/jphysiol.1953.sp004849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Morlock M. L., Benamy D. A., Grundfest H. Analysis of spike electrogenesis of Eel electroplaques with phase plane and impedance measurements. J Gen Physiol. 1968 Jul;52(1):22–45. doi: 10.1085/jgp.52.1.22. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nakamura Y., Nakajima S., Grundfest H. Analysis of Spike Electrogenesis and Depolarizing K Inactivation in Electroplaques of Electrophorus electricus, L. J Gen Physiol. 1965 Nov 1;49(2):321–349. doi: 10.1085/jgp.49.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Noble D., Tsien R. W. The kinetics and rectifier properties of the slow potassium current in cardiac Purkinje fibres. J Physiol. 1968 Mar;195(1):185–214. doi: 10.1113/jphysiol.1968.sp008454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ruiz-Manresa F., Ruarte A. C., Schwartz T. L., Grundfest H. Potassium inactivation and impedance changes during spike electrogenesis in eel electroplaques. J Gen Physiol. 1970 Jan;55(1):33–47. doi: 10.1085/jgp.55.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SCHOFFENIELS E. Electrical activity of isolated single electroplax of electric eel as affected by temperature. Science. 1958 May 9;127(3306):1117–1118. doi: 10.1126/science.127.3306.1117. [DOI] [PubMed] [Google Scholar]
  14. Ulbricht W. Effect of temperature on the slowly changing sodium permeability of veratrinized nodes of Ranvier. Pflugers Arch. 1969;311(1):73–95. doi: 10.1007/BF00588063. [DOI] [PubMed] [Google Scholar]
  15. WITT P. N., SWAINE C. R. Studies on Veratrum alkaloids. XXV. Veratrine response and antiveratrinic action in frog sartorius muscle. J Pharmacol Exp Ther. 1957 May;120(1):63–74. [PubMed] [Google Scholar]

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