Abstract
Our previous studies have shown that, while a persistent but potentially reversible suppression of the tumorous state appears to be a characteristic feature of the vegetative phase of teratoma shoot growth in the crown gall disease of plants, a recovery from that state occurs during the reproductive phase. An analysis has now been made of the reproductive process in an attempt to define the precise stage at which recovery occurs. The results of this analysis have shown that diploid somatic cells of teratoma-derived flower parts such as those found in petals and filaments are inherently neoplastic. On the other hand, haploid cells of plants grown from anthers of the same flowers and diploid cells of F1 generation plants grown from teratoma-derived seed have, by generally accepted criteria, recovered from the tumorous state. These findings have been interpreted to mean that the loss of neoplastic properties occurs in crown gall teratoma cells during meiosis rather than during fertilization or later stages of the reproductive process.
An analysis of more than 2000 teratoma-derived tumor shoots has shown, moreover, that a recovery from the tumorous state may also occur, although apparently as a very rare event, during the vegetative phase of teratoma shoot growth.
Keywords: teratoma, meiosis, vegetative propagation, nopaline
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aaron-da Cunha M. I. Sur la libération, par les rayons x, d'un principe tumorigène contenu dans les tissus de crown gall de tabac. C R Acad Sci Hebd Seances Acad Sci D. 1969 Jan 13;268(2):318–321. [PubMed] [Google Scholar]
- Binns A., Meins F. Habituation of tobacco pith cells for factors promoting cell division is heritable and potentially reversible. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2660–2662. doi: 10.1073/pnas.70.9.2660. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Braun A. C. A DEMONSTRATION OF THE RECOVERY OF THE CROWN-GALL TUMOR CELL WITH THE USE OF COMPLEX TUMORS OF SINGLE-CELL ORIGIN. Proc Natl Acad Sci U S A. 1959 Jul;45(7):932–938. doi: 10.1073/pnas.45.7.932. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Braun A. C., Wood H. N. Suppression of the neoplastic state with the acquisition of specialized functions in cells, tissues, and organs of crown gall teratomas of tobacco. Proc Natl Acad Sci U S A. 1976 Feb;73(2):496–500. doi: 10.1073/pnas.73.2.496. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carlson P. S., Smith H. H., Dearing R. D. Parasexual interspecific plant hybridization. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2292–2294. doi: 10.1073/pnas.69.8.2292. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frankel A. S., Haapala D. K., Neubauer R. L., Fischinger P. J. Elimination of the sarcoma genome from murine sarcoma virus transformed cat cells. Science. 1976 Mar 26;191(4233):1264–1266. doi: 10.1126/science.1257745. [DOI] [PubMed] [Google Scholar]
- Nitsch J. P., Nitsch C. Haploid plants from pollen grains. Science. 1969 Jan 3;163(3862):85–87. doi: 10.1126/science.163.3862.85. [DOI] [PubMed] [Google Scholar]