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. 1977 Jul;74(7):2720–2724. doi: 10.1073/pnas.74.7.2720

A DNA fragment containing the origin of replication of the Escherichia coli chromosome.

R C Marsh, A Worcel
PMCID: PMC431261  PMID: 268621

Abstract

A 38 kilobase pair region of the Escherichia coli K12 chromosome containing the replication origin has been physically mapped with restriction endonucleases EcoRI and HindIII. Replication starts within or very near a 1.3 kilobase pair HindIII fragment in the middle of this region and proceeds outward in both directions with apparently equal speed. This pattern was observed in both dnaA and dnaC temperature-sensitive (ts) initiation mutants at the start of the synchronous round of replication which occurs after downshift from the nonpermissive to the permissive temperature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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