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. 1976 Nov;73(11):3961–3965. doi: 10.1073/pnas.73.11.3961

Purification and characterization of a putative sigma factor from Chalamydomonas reinhardi.

S J Surzycki, D L Shellenbarger
PMCID: PMC431283  PMID: 792879

Abstract

Two proteins with sigma-like activity have been isolated from the alga, Chlamydomonas reinhardi. One protein, sigma 2, has been partially purified and appears to have a molecular weight of 51,000. The interaction of this protein with a heterologous (Escherichia coli) and homologous (Chlamydomonas, chloroplast rifampicin-sensitive) core RNA-polymerase (RNA nucleotidyltransferase, nucleosidetriphosphate: RNA nucleotidyltransferase, EC 2.7.7.6) was studied. Sigma 2 protein appears to stimulate the formation of open (rapid starting) binary complexes by both of the core enzymes. Stimulation of transcription by sigma 2 on chloroplast DNA was greater when Chlamydomonas core enzyme was used. Moreover, in vitro transcription on a variety of templates using RNA polymerases I and II from Chlamydomonas was not stimulated by this protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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