Abstract
Sonicated 1,2-dihexadecyl-sn-glycero-3-phosphorylcholine forms liposomes. Studies by Fourier transform proton magnetic resonance of the interaction of these bilayers with some general anesthetics, i.e., chloroform, halothane, methoxyflurane, and enflurane, show that the addition of a general anesthetic to the liposomes and raising the temperature have a similar effect in cuasing the fluidization of the bilayer. General anesthetics act on the hydrophilic site (choline group) in clinical concentrations and then diffuse into the hydrophobic region with the addition of larger amount of anesthetics. There is evidence that the lecithin choline groups are involved in the interaction with protein and that the general anesthetics change the conformation of some polypeptides and proteins. We conclude that the general anesthetics, by increasing the motion of positively charged choline groups and negatively charged groups in protein, weaken the Coulomb-type interaction and cause the liprotein conformational changes.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chan S. I., Sheetz M. P., Seiter C. H., Feigenson G. W., Hsu M. C., Lau A., Yau A. Nuclear magnetic resonance studies of the structure of model membrane systems: the effect of surface curvature. Ann N Y Acad Sci. 1973 Dec 31;222:499–522. doi: 10.1111/j.1749-6632.1973.tb15283.x. [DOI] [PubMed] [Google Scholar]
- Chang C. A., Chan S. I. Nuclear magnetic resonance studies of the interactions of sonicated lecithin bilayers with poly (L-glutamic acid). Biochemistry. 1974 Oct 8;13(21):4381–4385. doi: 10.1021/bi00718a022. [DOI] [PubMed] [Google Scholar]
- Chapman D. Nuclear magnetic resonance spectroscopic studies of biological membranes. Ann N Y Acad Sci. 1972 Jun 20;195:179–206. [PubMed] [Google Scholar]
- Davis D. G., Inesi G. Phosphorus and proton nuclear magnetic resonance studies in sarcoplasmic reticulum membranes and lipids. A comparison of phosphate and proton group mobilities in membranes and lipid bilayers. Biochim Biophys Acta. 1972 Sep 1;282(1):180–186. doi: 10.1016/0005-2736(72)90322-7. [DOI] [PubMed] [Google Scholar]
- Finer E. G., Flook A. G., Hauser H. Mechanism of sonication of aqueous egg yolk lecithin dispersions and nature of the resultant particles. Biochim Biophys Acta. 1972 Jan 27;260(1):49–58. doi: 10.1016/0005-2760(72)90073-2. [DOI] [PubMed] [Google Scholar]
- Hauser H. O. The effect of ultrasonic irradiation on the chemical structure of egg lecithin. Biochem Biophys Res Commun. 1971 Nov;45(4):1049–1055. doi: 10.1016/0006-291x(71)90443-8. [DOI] [PubMed] [Google Scholar]
- Horwitz A. F., Michaelson D., Klein M. P. Magnetic resonance studies on membrane and model membrane systems. 3. Fatty acid motions in aqueous lecithin dispersions. Biochim Biophys Acta. 1973 Feb 27;298(1):1–7. doi: 10.1016/0005-2736(73)90002-3. [DOI] [PubMed] [Google Scholar]
- Huang C. Studies on phosphatidylcholine vesicles. Formation and physical characteristics. Biochemistry. 1969 Jan;8(1):344–352. doi: 10.1021/bi00829a048. [DOI] [PubMed] [Google Scholar]
- Robinson J. D., Birdsall N. J., Lee A. G., Metcalfe J. C. 13 C and 1 H nuclear magnetic resonance relaxation measurements of the lipids of sarcoplasmic reticulum membranes. Biochemistry. 1972 Jul 18;11(15):2903–2909. doi: 10.1021/bi00765a025. [DOI] [PubMed] [Google Scholar]
- Sheetz M. P., Chan S. I. Effect of sonication on the structure of lecithin bilayers. Biochemistry. 1972 Nov 21;11(24):4573–4581. doi: 10.1021/bi00774a024. [DOI] [PubMed] [Google Scholar]