Abstract
Two fast-sedimenting chromatin complexes with sedimentation velocities of approximately 4600 and 3000 S can be isolated from logarithmically growing diploid Saccaromyces cerevisiae cells. The DNA in both structures appears to be folded into at least 60 domains and characterized by a negative superhelical density. Sensitivity to proteases and insensitivity to RNases suggest that proteins and not RNA are important in maintaining the organization of the chromosomes in both structures. The 46000S and 3000S complexes represent folded genomes isolated from diploid cells in the G2 and G1 stages of the cell cycle, respectively.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aaronson R. P., Blobel G. Isolation of nuclear pore complexes in association with a lamina. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1007–1011. doi: 10.1073/pnas.72.3.1007. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Benyajati C., Worcel A. Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogaster. Cell. 1976 Nov;9(3):393–407. doi: 10.1016/0092-8674(76)90084-2. [DOI] [PubMed] [Google Scholar]
- Berezney R., Coffey D. S. Nuclear protein matrix: association with newly synthesized DNA. Science. 1975 Jul 25;189(4199):291–293. doi: 10.1126/science.1145202. [DOI] [PubMed] [Google Scholar]
- Bicknell J. N., Douglas H. C. Nucleic acid homologies among species of Saccharomyces. J Bacteriol. 1970 Feb;101(2):505–512. doi: 10.1128/jb.101.2.505-512.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CUMMINGS D. J. SEDIMENTATION AND BIOLOGICAL PROPERTIES OF T-PHAGES OF ESCHERICHIA COLI. Virology. 1964 Jul;23:408–418. doi: 10.1016/0042-6822(64)90264-8. [DOI] [PubMed] [Google Scholar]
- Cook P. R., Brazell I. A. Supercoils in human DNA. J Cell Sci. 1975 Nov;19(2):261–279. doi: 10.1242/jcs.19.2.261. [DOI] [PubMed] [Google Scholar]
- Germond J. E., Hirt B., Oudet P., Gross-Bellark M., Chambon P. Folding of the DNA double helix in chromatin-like structures from simian virus 40. Proc Natl Acad Sci U S A. 1975 May;72(5):1843–1847. doi: 10.1073/pnas.72.5.1843. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hancock R. Interphase chromosomal deoxyribonucleoprotein isolated as a discrete structure from cultured cells. J Mol Biol. 1974 Jul 5;86(3):649–663. doi: 10.1016/0022-2836(74)90187-9. [DOI] [PubMed] [Google Scholar]
- Hutchinson F., Krasin F. Dependence of the sedimentation of high molecular weight DNA on centrifuge speed. Biophys Chem. 1976 Dec;6(1):23–29. doi: 10.1016/0301-4622(76)80058-0. [DOI] [PubMed] [Google Scholar]
- Keller J. M., Riley D. E. Nuclear ghosts: a nonmembranous structural component of mammalian cell nuclei. Science. 1976 Jul 30;193(4251):399–401. doi: 10.1126/science.935874. [DOI] [PubMed] [Google Scholar]
- Kornberg R. D. Chromatin structure: a repeating unit of histones and DNA. Science. 1974 May 24;184(4139):868–871. doi: 10.1126/science.184.4139.868. [DOI] [PubMed] [Google Scholar]
- Lauer G. D., Klotz L. C. Determination of the molecular weight of Saccharomyces cerevisiae nuclear DNA. J Mol Biol. 1975 Jun 25;95(2):309–326. doi: 10.1016/0022-2836(75)90397-6. [DOI] [PubMed] [Google Scholar]
- LePecq J. B., Paoletti C. A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. J Mol Biol. 1967 Jul 14;27(1):87–106. doi: 10.1016/0022-2836(67)90353-1. [DOI] [PubMed] [Google Scholar]
- Levin D., Hutchinson F. Neutral sucrose sedimentation of very large DNA from Bacillus subtilis. I. Effect of random double-strand breaks and centrifuge speed on sedimentation. J Mol Biol. 1973 Apr 15;75(3):455–478. doi: 10.1016/0022-2836(73)90454-3. [DOI] [PubMed] [Google Scholar]
- Levin D., Hutchinson F. Relation between single-strand DNA mass and sedimentation distance in alkaline sucrose gradients. J Mol Biol. 1973 Apr 15;75(3):495–502. doi: 10.1016/0022-2836(73)90456-7. [DOI] [PubMed] [Google Scholar]
- Lohr D., Van Holde K. E. Yeast chromatin subunit structure. Science. 1975 Apr 11;188(4184):165–166. doi: 10.1126/science.1090006. [DOI] [PubMed] [Google Scholar]
- Olins A. L., Olins D. E. Spheroid chromatin units (v bodies). Science. 1974 Jan 25;183(4122):330–332. doi: 10.1126/science.183.4122.330. [DOI] [PubMed] [Google Scholar]
- Oudet P., Gross-Bellard M., Chambon P. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit. Cell. 1975 Apr;4(4):281–300. doi: 10.1016/0092-8674(75)90149-x. [DOI] [PubMed] [Google Scholar]
- Petes T. D., Fangman W. L. Sedimentation properties of yeast chromosomal DNA. Proc Natl Acad Sci U S A. 1972 May;69(5):1188–1191. doi: 10.1073/pnas.69.5.1188. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Piñon R., Salts Y., Simchen G. Nuclear and mitochondrial DNA synthesis during yeast sporulation. Exp Cell Res. 1974 Feb;83(2):231–238. doi: 10.1016/0014-4827(74)90334-6. [DOI] [PubMed] [Google Scholar]
- Ryder O. A., Smith D. W. Properties of membrane-associated folded chromosomes of E. coli related to initiation and termination of DNA replication. Cell. 1975 Apr;4(4):337–345. doi: 10.1016/0092-8674(75)90154-3. [DOI] [PubMed] [Google Scholar]
- Sekine H., Nakano E., Sakaguchi K. The interaction of DNA with pancreatic ribonuclease A. Biochim Biophys Acta. 1969 Jan 21;174(1):202–210. doi: 10.1016/0005-2787(69)90243-3. [DOI] [PubMed] [Google Scholar]
- Simchen G., Piñon R., Salts Y. Sporulation in Saccharomyces cerevisiae: premeiotic DNA synthesis, readiness and commitment. Exp Cell Res. 1972 Nov;75(1):207–218. doi: 10.1016/0014-4827(72)90538-1. [DOI] [PubMed] [Google Scholar]
- Thomas J. O., Furber V. Yeast chromatin structure. FEBS Lett. 1976 Jul 15;66(2):274–280. doi: 10.1016/0014-5793(76)80521-2. [DOI] [PubMed] [Google Scholar]
- Wang J. C. Degree of superhelicity of covalently closed cyclic DNA's from Escherichia coli. J Mol Biol. 1969 Jul 28;43(2):263–272. doi: 10.1016/0022-2836(69)90266-6. [DOI] [PubMed] [Google Scholar]
- Worcel A., Burgi E. On the structure of the folded chromosome of Escherichia coli. J Mol Biol. 1972 Nov 14;71(2):127–147. doi: 10.1016/0022-2836(72)90342-7. [DOI] [PubMed] [Google Scholar]
- Worcel A., Burgi E. Properties of a membrane-attached form of the folded chromosome of Escherichia coli. J Mol Biol. 1974 Jan 5;82(1):91–105. doi: 10.1016/0022-2836(74)90576-2. [DOI] [PubMed] [Google Scholar]