Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Jul;74(7):2963–2967. doi: 10.1073/pnas.74.7.2963

Recombinant plasmid that carries part of the nitrogen fixation (nif) gene cluster of Klebsiella pneumoniae.

F C Cannon, G E Riedel, F M Ausubel
PMCID: PMC431364  PMID: 331321

Abstract

We have cloned fragments of the Klebsiella pneumoniae genome that carry part of the his operon and part of the nitrogen fixation (nif) gene cluster on the amplifiable plasmid pMB9. One particular plasmid, pCRA37, complements mutations in the hisD, nifB, and nifF loci. The physical map of pCRA37 as determined by restriction enzyme analysis correlates with the genetic map of the his-nif region as determined previously by phage P1-mediated cotransductional analysis.

Full text

PDF

Images in this article

2965Image
on p.2965
2965Image
on p.2965

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachhuber M., Brill W. J., Howe M. M. Use of bacteriophage Mu to isolate deletions in the his-nif region of Klebsiella pneumoniae. J Bacteriol. 1976 Dec;128(3):749–753. doi: 10.1128/jb.128.3.749-753.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bedbrook J. R., Ausubel F. M. Recombination between bacterial plasmids leading to the formation of plasmid multimers. Cell. 1976 Dec;9(4 Pt 2):707–716. doi: 10.1016/0092-8674(76)90134-3. [DOI] [PubMed] [Google Scholar]
  3. Bedbrook J. R., Bogorad L. Endonuclease recognition sites mapped on Zea mays chloroplast DNA. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4309–4313. doi: 10.1073/pnas.73.12.4309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cannon F. C., Dixon R. A., Postgate J. R. Derivation and properties of F-prime factors in Escherichia coli carrying nitrogen fixation genes from Klebsiella pneumoniae. J Gen Microbiol. 1976 Mar;93(1):111–125. doi: 10.1099/00221287-93-1-111. [DOI] [PubMed] [Google Scholar]
  5. Cannon F. C., Dixon R. A., Postgate J. R., Primrose S. B. Chromosomal integration of Klebsiella nitrogen fixation genes in Escherichia coli. J Gen Microbiol. 1974 Jan;80(1):227–239. doi: 10.1099/00221287-80-1-227. [DOI] [PubMed] [Google Scholar]
  6. Cannon F. C., Dixon R. A., Postgate J. R., Primrose S. B. Plasmids formed in nitrogen-fixing Escherichia coli-Klebsiella pneumoniae hybrids. J Gen Microbiol. 1974 Jan;80(1):241–251. doi: 10.1099/00221287-80-1-241. [DOI] [PubMed] [Google Scholar]
  7. Cannon F. C., Postgate J. R. Expression of Klebsiella nitrogen fixation genes (nif) in Azotobacter. Nature. 1976 Mar 18;260(5548):271–272. doi: 10.1038/260271a0. [DOI] [PubMed] [Google Scholar]
  8. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Clewell D. B. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. doi: 10.1128/jb.110.2.667-676.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cohen S. N., Chang A. C., Boyer H. W., Helling R. B. Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240–3244. doi: 10.1073/pnas.70.11.3240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Dixon R. A., Postgate J. R. Transfer of nitrogen-fixation genes by conjugation in Klebsiella pneumoniae. Nature. 1971 Nov 5;234(5323):47–48. doi: 10.1038/234047a0. [DOI] [PubMed] [Google Scholar]
  13. Dixon R., Cannon F., Kondorosi A. Construction of a P plasmid carrying nitrogen fixation genes from Klebsiella pneumoniae. Nature. 1976 Mar 18;260(5548):268–271. doi: 10.1038/260268a0. [DOI] [PubMed] [Google Scholar]
  14. Dugaiczyk A., Boyer H. W., Goodman H. M. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures. J Mol Biol. 1975 Jul 25;96(1):171–184. doi: 10.1016/0022-2836(75)90189-8. [DOI] [PubMed] [Google Scholar]
  15. Garrick-Silversmith L., Hartman P. E. Histidine-requiring mutants of Escherichia coli K12. Genetics. 1970 Oct;66(2):231–244. doi: 10.1093/genetics/66.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Goldschmidt E. P., Cater M. S., Matney T. S., Butler M. A., Greene A. Genetic analysis of the histidine operon in Escherichia coli K12. Genetics. 1970 Oct;66(2):219–229. doi: 10.1093/genetics/66.2.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hayward G. S., Smith M. G. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis. J Mol Biol. 1972 Feb 14;63(3):383–395. doi: 10.1016/0022-2836(72)90435-4. [DOI] [PubMed] [Google Scholar]
  18. Hershfield V., Boyer H. W., Yanofsky C., Lovett M. A., Helinski D. R. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455–3459. doi: 10.1073/pnas.71.9.3455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Maizels N. Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit. Cell. 1976 Nov;9(3):431–438. doi: 10.1016/0092-8674(76)90088-x. [DOI] [PubMed] [Google Scholar]
  20. Nosikov V. V., Braga E. A., Karlishev A. V., Zhuze A. L., Polyanovsky O. L. Protection of particular cleavage sites of restriction endonucleases by distamycin A and actinomycin D. Nucleic Acids Res. 1976 Sep;3(9):2293–2301. doi: 10.1093/nar/3.9.2293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Panet A., van de Sande J. H., Loewen P. C., Khorana H. G., Raae A. J., Lillehaug J. R., Kleppe K. Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase. Biochemistry. 1973 Dec 4;12(25):5045–5050. doi: 10.1021/bi00749a003. [DOI] [PubMed] [Google Scholar]
  22. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  23. St John R. T., Johnston H. M., Seidman C., Garfinkel D., Gordon J. K., Shah V. K., Brill W. J. Biochemistry and genetics of Klebsiella pneumoniae mutant strains unable to fix N2. J Bacteriol. 1975 Mar;121(3):759–765. doi: 10.1128/jb.121.3.759-765.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Streicher S. L., Gurney E. G., Valentine R. C. The nitrogen fixation genes. Nature. 1972 Oct 27;239(5374):495–499. doi: 10.1038/239495a0. [DOI] [PubMed] [Google Scholar]
  25. Streicher S., Gurney E., Valentine R. C. Transduction of the nitrogen-fixation genes in Klebsiella pneumoniae. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1174–1177. doi: 10.1073/pnas.68.6.1174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tanaka T., Weisblum B. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J Bacteriol. 1975 Jan;121(1):354–362. doi: 10.1128/jb.121.1.354-362.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Thomas M., Davis R. W. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975 Jan 25;91(3):315–328. doi: 10.1016/0022-2836(75)90383-6. [DOI] [PubMed] [Google Scholar]
  28. Wu T. T. A model for three-point analysis of random general transduction. Genetics. 1966 Aug;54(2):405–410. doi: 10.1093/genetics/54.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES