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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Nov;73(11):4169–4173. doi: 10.1073/pnas.73.11.4169

Localization of gene functions in polyoma virus DNA.

J Feunteun, L Sompayrac, M Fluck, T Benjamin
PMCID: PMC431371  PMID: 186787

Abstract

Polyoma virus mutants of four functionally distinct groups have been mapped by the marker rescue technique using restriction enzyme fragments of wild-type viral DNA. Nontransforming host-range mutants map in the proximal part of the early region of the viral genome. The same DNA fragment that restores a normal host range also restores normal transforming ability to these mutants. ts-25D, a temperature-sensitive (ts)-a class mutant, maps in the distal part of the early region. ts-3 and ts-1260 map in the proximal and distal parts of the late region, respectively.

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Selected References

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  1. Barzilai R. SV40 DNA: quantitative conversion of closed circular to open circular form by an ethidium bromide-restricted endonuclease. J Mol Biol. 1973 Mar 15;74(4):739–742. doi: 10.1016/0022-2836(73)90062-4. [DOI] [PubMed] [Google Scholar]
  2. Benjamin T. L. Host range mutants of polyoma virus. Proc Natl Acad Sci U S A. 1970 Sep;67(1):394–399. doi: 10.1073/pnas.67.1.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Di Mayorca G., Callender J., Marin G., Giordano R. Temperature-sensitive mutants of polyoma virus. Virology. 1969 May;38(1):126–133. doi: 10.1016/0042-6822(69)90134-2. [DOI] [PubMed] [Google Scholar]
  4. Echkart W. Genetics of DNA tumor viruses. Annu Rev Genet. 1974;8:301–317. doi: 10.1146/annurev.ge.08.120174.001505. [DOI] [PubMed] [Google Scholar]
  5. Eckhart W. Complementation and transformation by temperature-sensitive mutants of polyoma virus. Virology. 1969 May;38(1):120–125. doi: 10.1016/0042-6822(69)90133-0. [DOI] [PubMed] [Google Scholar]
  6. Eckhart W., Dulbecco R. Properties of the ts3 mutant of polyoma virus during lytic infection. Virology. 1974 Aug;60(2):359–369. doi: 10.1016/0042-6822(74)90331-6. [DOI] [PubMed] [Google Scholar]
  7. Goldman E., Benjamin T. L. Analysis of host range of nontransforming polyoma virus mutants. Virology. 1975 Aug;66(2):372–384. doi: 10.1016/0042-6822(75)90210-x. [DOI] [PubMed] [Google Scholar]
  8. Hamer D. H., Thomas C. A., Jr Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI. Proc Natl Acad Sci U S A. 1976 May;73(5):1537–1541. doi: 10.1073/pnas.73.5.1537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  10. Hutchison C. A., 3rd, Edgell M. H. Genetic assay for small fragments of bacteriophage phi X174 deoxyribonucleic acid. J Virol. 1971 Aug;8(2):181–189. doi: 10.1128/jvi.8.2.181-189.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kamen R., Lindstrom D. M., Shure H., Old R. W. Virus-specific RNA in cells productively infected or transformed by polyoma virus. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):187–198. doi: 10.1101/sqb.1974.039.01.025. [DOI] [PubMed] [Google Scholar]
  12. Lai C. J., Nathans D. A map of temperature-sensitive mutants of simian virus 40. Virology. 1975 Jul;66(1):70–81. doi: 10.1016/0042-6822(75)90179-8. [DOI] [PubMed] [Google Scholar]
  13. Lai C. J., Nathans D. Mapping temperature-sensitive mutants of simian virus 40: rescue of mutants by fragments of viral DNA. Virology. 1974 Aug;60(2):466–475. doi: 10.1016/0042-6822(74)90340-7. [DOI] [PubMed] [Google Scholar]
  14. MACPHERSON I., MONTAGNIER L. AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS. Virology. 1964 Jun;23:291–294. doi: 10.1016/0042-6822(64)90301-0. [DOI] [PubMed] [Google Scholar]
  15. Mantel N., Boyer H. W., Goodman H. M. Mapping simian virus 40 mutants by construction of partial heterozygotes. J Virol. 1975 Sep;16(3):754–757. doi: 10.1128/jvi.16.3.754-757.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Miller L. K., Fried M. Construction of infectious polyoma hybrid genomes in vitro. Nature. 1976 Feb 19;259(5544):598–601. doi: 10.1038/259598a0. [DOI] [PubMed] [Google Scholar]
  17. Miller L. K., Fried M. Construction of the genetic map of the polyoma genome. J Virol. 1976 Jun;18(3):824–832. doi: 10.1128/jvi.18.3.824-832.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Oxman M. N., Takemoto K. K., Eckhart W. Polyoma T antigen synthesis by temperature-sensitive mutants of polyoma virus. Virology. 1972 Sep;49(3):675–682. doi: 10.1016/0042-6822(72)90524-7. [DOI] [PubMed] [Google Scholar]
  19. Paulin D., Cuzin F. Polyoma virus T antigen. I. Synthesis of modified heat-labile T angiten in cells transformed with the ts-a mutant. J Virol. 1975 Feb;15(2):393–397. doi: 10.1128/jvi.15.2.393-397.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schaffhausen B. S., Benjamin T. L. Deficiency in histone acetylation in nontransforming host range mutants of polyoma virus. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1092–1096. doi: 10.1073/pnas.73.4.1092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  22. Shenk T. E., Carbon J., Berg P. Construction and analysis of viable deletion mutants of simian virus 40. J Virol. 1976 May;18(2):664–671. doi: 10.1128/jvi.18.2.664-671.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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