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. 1976 Nov;73(11):4174–4178. doi: 10.1073/pnas.73.11.4174

Construction of plasmids carrying the cI gene of bacteriophage lambda.

K Backman, M Ptashne, W Gilbert
PMCID: PMC431373  PMID: 1069307

Abstract

By techniques of recombination in vitro, we have constructed a plasmid bearing the repressor gene (cI) of bacteriophage lambda fused to the promoter of the lac operon. Strains carrying this plasmid overproduce lambda repressor. This functional cI gene was reconstituted by joining DNA fragments bearing different parts of that gene. Flush end fusion techniques, involving no sequence overlap, were necessary for the construction; in certain cases, the abutting of the DNA molecules bearing ends generated by different restriction endonucleases creates a sequence at the junction which is recognized by one of the restriction endonucleases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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