Correction: The Tumor Suppressor Gene, RASSF1A, Is Essential for Protection against Inflammation -Induced Injury

There is an error in the legend for Fig. 8. Please see the corrected Fig. 8 here.

Figure 8. The PTK inhibitor, imatinib, inhibits the appearance of pY-YAP and promoted increased survival of Rassf1a^+/− but not Rassf1a^−/− mice animals following inflammation-induced injury.

(A) Rassf1a^+/− or Rassf1a^−/− mice were intraperitoneally injected with the PTK inhibitor, imatinib at 60 mg/kg body weight on days 3 and 6 following 3% DSS addition. P-value between survival of DSS-treated wild type and Rassf1a^+/− was <0.0001 (n = 17) and between DSS-treated Rassf1a^+/− (+ imatinib) versus DSS-treated Rassf1a^+/− was 0.0086 (n = 17). No significance difference was observed between DSS-treated Rassf1a^+/− and DSS-treated Rassf1a^−/− (+ imatinib) mice (please see Fig. 1A for the survival curve of DSS-treated Rassf1a^−/− mice). Following DSS/gleevec treatment, (B) histological analysis of colonic sections, (C) serum IL-6, (D) cell death via PARP (late marker of apoptosis); (E) phospho-YAP by IHC, and (F) In vitro kinase activity was carried out for c-Abl using colon lystes from DSS-treated wild type and Rassf1a^+/− (top panel) and Rassf1a^−/− (bottom panel) mice with overexpressed FLAG-YAP as substrate. Expression levels of c-Abl were similar in all the lanes (data not shown) and bacterially expressed GST or GST-1A (1A) was used to explore how RASSF1A may directly interfere with c-Abl kinase activity. Expression of FLAG-YAP, GST and GST-1A are shown in Fig. S7D. For (B) p-value between wild type versus Rassf1a^+/− mice (+DSS) was 0.004, wild type versus Rassf1a^+/− mice + DSS + gleevec) was 0.168 and wild type versus Rassf1a^−/− mice (+DSS + gleevec) was 0.452 (n = 4 – 8). For (C), p-value between wild type versus Rassf1a^+/− mice (+DSS) was 0.004 and wild type versus Rassf1a^+/− mice (+ DSS + gleevec) was 0.347 and wild type versusRassf1a^−/− mice (+DSS + gleevec) was 0.262 (n = 4 – 8). For (E) P values of Rassf1a^+/− mice orRassf1a^−/− mice (+DSS −/+ gleevec) was <0.001 (n = 10).