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. 1977 Jul;74(7):3047–3051. doi: 10.1073/pnas.74.7.3047

Authentic beta-globin mRNA sequences in homozygous betaO-thalassemia.

G F Temple, J C Chang, Y W Kan
PMCID: PMC431405  PMID: 268654

Abstract

In a patient with homozygous betaO-thalassemia in whom studies of reticulocyte hemoglobin synthesis showed no beta-globin chain synthesis in vivo and in vitro, molecular hybridization studies revealed RNA sequences complementary to beta-globin cDNA. The fact that these sequences were authentic beta-globin mRNA was shown by fingerprint analysis of T1 ribonuclease-digested mRNA and by sequencing of oligonucleotides unique to beta-globin mRNA. The beta-mRNA that failed to direct beta-globin chain synthesis was not detectably shortened or degraded and contained poly(A) sequences.

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Selected References

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