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. 1977 Aug;74(8):3217–3221. doi: 10.1073/pnas.74.8.3217

DNA-directed synthesis in vitro of beta-galactosidase: requirement for a ribosome release factor.

H F Kung, B V Treadwell, C Spears, P C Tai, H Weissbach
PMCID: PMC431503  PMID: 410018

Abstract

The DNA-directed synthesis of beta-galactosidase in Escherichia coli extracts has been investigated in a partially fractionated system. A dependency was obtained for 3',5'-cyclic AMP receptor protein and also for a factor, from the salt wash of ribosomes, that has been purified to near homogeneity. This factor has been identified with a ribosome release factor previously purified from the supernatant fraction by A. Hirashima and A. Kaji [(1972) Biochemistry 11,4037-4044]. In the coupled transcription-translation system this factor stimulates beta-galactosidase synthesis and total protein synthesis 2- to 4-fold. It is thus clear that the ribosome release factor has a physiological function in translation. It may also affect transcription, because it stimulated total RNA synthesis up to 50% in this in vitro system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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