Fig. 1.

Schematic representation of the PPRV recombinant plasmids and predicted epitopes for C77 monoclonal antibody binding. (a) The synthetic plasmid, pPPRV+GFP, incorporating eGFP as a reporter gene between the P and M genes and restriction enzyme sequences. (b) Plasmid, pPPRV without the eGFP gene incorporating restriction enzyme sequences. (c) Plasmid, pPPRV-C77 incorporating mutations at R547 S549 S550 amino acid positions on the H gene. (d) The proposed epitope (upper strand) for C77 monoclonal antibody binding as determined by phage display, and the mutated H (lower strand). Residues critical to C77 binding are highlighted in grey and indicated by star symbol. Amino acid residues mutated to alanine in plasmid pPPRV-C77 are boldfaced and underlined in lower strand.