Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Aug;74(8):3230–3234. doi: 10.1073/pnas.74.8.3230

Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA.

J Ghysdael, E Hubert, M Trávnícek, D P Bolognesi, A Burny, Y Cleuter, G Huez, R Kettmann, G Marbaix, D Portetelle, H Chantrenne
PMCID: PMC431509  PMID: 198776

Abstract

After microinjection of Xenopus laevis oocytes with RNA from avian myeloblastosis virus, viral structural proteins p27, p19, p15, and p12 are formed by a sequence of posttranslational cleavages of a high-molecular-weight precursor polypeptide. The 60-70S RNA aggregate or its 30-40S RNA subunits obtained by heat or formamide treatment possess the same ability to serve as template in X. laevis oocytes. The processing pattern of virus-specific precursor polypeptides is the same in X. laevis oocytes as in chick embryo fibroblasts infected with avian myeloblastosis virus, but the processing takes place at a much slower rate.

Full text

PDF
3230

Images in this article

3231Image
on p.3231
3232Image
on p.3232
3232Image
on p.3232
3233Image
on p.3233

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. C. A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology. 1974 Aug;60(2):595–600. doi: 10.1016/0042-6822(74)90356-0. [DOI] [PubMed] [Google Scholar]
  2. Berns A. J., van Kraaikamp M., Bloemendal H., Lane C. D. Calf crystallin synthesis in frog cells: the translation of lens-cell 14S RNA in oocytes. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1606–1609. doi: 10.1073/pnas.69.6.1606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berridge M. V., Lane C. D. Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins. Cell. 1976 Jun;8(2):283–297. doi: 10.1016/0092-8674(76)90012-x. [DOI] [PubMed] [Google Scholar]
  4. Bishop D. H., Ruprecht R., Simpson R. W., Spiegelman S. Deoxyribonucleic acid polymerase of Rous sarcoma virus: reaction conditions and analysis of the reaction product nucleic acids. J Virol. 1971 Nov;8(5):730–741. doi: 10.1128/jvi.8.5.730-741.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boime I., Leder P. Protein synthesis directed by encephalomyocarditis virus mRNA. 3. Discrete polypeptides translated from a monocistronic messenger in vitro. Arch Biochem Biophys. 1972 Dec;153(2):706–713. doi: 10.1016/0003-9861(72)90389-x. [DOI] [PubMed] [Google Scholar]
  6. Bolognesi D. P., Ishizaki R., Hüper G., Vanaman T. C., Smith R. E. Immunological properties of avian oncornavirus polypeptides. Virology. 1975 Apr;64(2):349–357. doi: 10.1016/0042-6822(75)90111-7. [DOI] [PubMed] [Google Scholar]
  7. Eisenman R., Vogt V. M., Diggelmann H. Synthesis of avian RNA tumor virus structural proteins. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1067–1075. doi: 10.1101/sqb.1974.039.01.122. [DOI] [PubMed] [Google Scholar]
  8. Gurdon J. B., Lane C. D., Woodland H. R., Marbaix G. Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature. 1971 Sep 17;233(5316):177–182. doi: 10.1038/233177a0. [DOI] [PubMed] [Google Scholar]
  9. Kerr I. M., Olshevsky U., Lodish H. F., Baltimore D. Translation of murine leukemia virus RNA in cell-free systems from animal cells. J Virol. 1976 May;18(2):627–635. doi: 10.1128/jvi.18.2.627-635.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kettmann R., Portetelle D., Mammerickx M., Cleuter Y., Dekegel D., Galoux M., Ghysdael J., Burny A., Chantrenne H. Bovine leukemia virus: an exogenous RNA oncogenic virus. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1014–1018. doi: 10.1073/pnas.73.4.1014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Laskey R. A., Gurdon J. B., Crawford L. V. Translation of encephalomyocarditis viral RNA in oocytes of Xenopus laevis. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3665–3669. doi: 10.1073/pnas.69.12.3665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  14. Naso R. B., Arcement L. J., Arlinghaus R. B. Biosynthesis of Rauscher leukemia viral proteins. Cell. 1975 Jan;4(1):31–36. doi: 10.1016/0092-8674(75)90130-0. [DOI] [PubMed] [Google Scholar]
  15. Naso R. B., Arcement L. J., Wood G., Saunders T. E., Arlinghaus R. B. The cell-free translation of Rauscher leukemia virus RNA into high molecular weight polypeptides. Biochim Biophys Acta. 1975 Mar 10;383(2):195–206. doi: 10.1016/0005-2787(75)90261-0. [DOI] [PubMed] [Google Scholar]
  16. Salden M., Asselbergs F., Bloemendal H. Translation of oncogenic virus RNA in Xenopus laevis oocytes. Nature. 1976 Feb 26;259(5545):696–699. doi: 10.1038/259696a0. [DOI] [PubMed] [Google Scholar]
  17. Shapiro S. Z., August J. T. Proteolytic cleavage events in oncornavirus protein synthesis. Biochim Biophys Acta. 1976 Dec 23;458(4):375–396. doi: 10.1016/0304-419x(76)90008-1. [DOI] [PubMed] [Google Scholar]
  18. Shapiro S. Z., Strand M., August J. T. High molecular weight precursor polypeptides to structural proteins of Rauscher murine leukemia virus. J Mol Biol. 1976 Nov 15;107(4):459–477. doi: 10.1016/s0022-2836(76)80078-2. [DOI] [PubMed] [Google Scholar]
  19. Trávnícek M., Ríman J. Subunits of oncornavirus high-molecular-weight RNA. I. Stepwise conversion of 60 S AMV (avian myeloblastosis virus) RNA to subunits. Biochem Biophys Res Commun. 1973 Jul 2;53(1):217–223. doi: 10.1016/0006-291x(73)91422-8. [DOI] [PubMed] [Google Scholar]
  20. Vogt V. M., Eisenman R., Diggelmann H. Generation of avian myeloblastosis virus structural proteins by proteolytic cleavage of a precursor polypeptide. J Mol Biol. 1975 Aug 15;96(3):471–493. doi: 10.1016/0022-2836(75)90174-6. [DOI] [PubMed] [Google Scholar]
  21. Vogt V. M., Eisenman R. Identification of a large polypeptide precursor of avian oncornavirus proteins. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1734–1738. doi: 10.1073/pnas.70.6.1734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zaanie D., Gielkens A. L., Dekker-michielsen M. J., Bloemers H. P. Virus-specific precursor polypeptides in cells infected with Rauscher leukemia virus. Virology. 1975 Oct;67(2):544–552. doi: 10.1016/0042-6822(75)90454-7. [DOI] [PubMed] [Google Scholar]
  23. von der Helm K. Cleavage of Rous sarcoma viral polypeptide precursor into internal structural proteins in vitro involves viral protein p15. Proc Natl Acad Sci U S A. 1977 Mar;74(3):911–915. doi: 10.1073/pnas.74.3.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. von der Helm K., Duesberg P. H. Translation of Rous sarcoma virus RNA in a cell-free system from ascites Krebs II cells. Proc Natl Acad Sci U S A. 1975 Feb;72(2):614–618. doi: 10.1073/pnas.72.2.614. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES