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. 1976 Dec;73(12):4487–4491. doi: 10.1073/pnas.73.12.4487

HLA membrane antigens: sequencing by intrinsic radioactivity.

B Ballou, D J McKean, E F Freedlender, O Smithies
PMCID: PMC431511  PMID: 794879

Abstract

Radiochemical sequence data are presented for the amino termini of mixed HLA antigens A1 and B8 isolated from a human lymphoblastoid cell line. Cells were labeled intrinsically in small-scale tissue culture with 14C-labeled amino acids and [14C]pyruvate. The specific activities obtained were sufficiently high and uniform to permit direct radiochemical sequencing of the antigens. HLA antigens were isolated by adsorption to a solid-phase anti-beta2-microglobulin immunoadsorbent followed by electrophoresis. The method should be generally applicable and useful for sequencing all parts of the molecule.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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