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. 1976 Dec;73(12):4506–4510. doi: 10.1073/pnas.73.12.4506

Innervation of heart cells in culture by an endogenous source of cholinergic neurons.

M A Lane, A Sastre, M M Salpeter
PMCID: PMC431519  PMID: 1070001

Abstract

Hearts of embryonic mice 9 days in utero were found to have an endogenous source of cholinergic neurons which can survive in dispersed cell cultures. These neurons are electrically excitable, have ultrastructural characteristics of cholinergic embryonic neurons, and functionally innervate heart cells in culture. The nature of the innervation described is muscarinic cholinergic.

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Selected References

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  1. BUNDY H. F., MEHL J. W. Trypsin inhibitors of human serum. II. Isolation of the alpha 1-inhibitor and its partial characterization. J Biol Chem. 1959 May;234(5):1124–1128. [PubMed] [Google Scholar]
  2. Betz W. Functional and non-functional contacts between ciliary neurones and muscle grown in vitro. J Physiol. 1976 Jan;254(1):75–86. doi: 10.1113/jphysiol.1976.sp011222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coraboeuf E., Le Douarin G., Obrecht-Coutris G. Release of acetylcholine by chick embryo heart before innervation. J Physiol. 1970 Feb;206(2):383–395. doi: 10.1113/jphysiol.1970.sp009019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coughlin M. D. Early development of parasympathetic nerves in the mouse submandibular gland. Dev Biol. 1975 Mar;43(1):123–139. doi: 10.1016/0012-1606(75)90136-0. [DOI] [PubMed] [Google Scholar]
  5. DeHann R. L. Regulation of spontaneous activity and growth of embryonic chick heart cells in tissue culture. Dev Biol. 1967 Sep;16(3):216–249. doi: 10.1016/0012-1606(67)90025-5. [DOI] [PubMed] [Google Scholar]
  6. Ertel R. J., Clarke D. E., Chao J. C., Franke F. R. Autonomic receptor mechanisms in embryonic chick myocardial cell cultures. J Pharmacol Exp Ther. 1971 Jul;178(1):73–80. [PubMed] [Google Scholar]
  7. Fischbach G. D. Synapse formation between dissociated nerve and muscle cells in low density cell cultures. Dev Biol. 1972 Jun;28(2):407–429. doi: 10.1016/0012-1606(72)90023-1. [DOI] [PubMed] [Google Scholar]
  8. HIGMAN H. B., PODLESKI T. R., BARTELS E. APPARENT DISSOCIATION CONSTANTS BETWEEN CARBAMYLCHOLINE, DELTA-TUBOCURARINE AND THE RECEPTOR. Biochim Biophys Acta. 1963 Sep 24;75:187–193. doi: 10.1016/0006-3002(63)90597-3. [DOI] [PubMed] [Google Scholar]
  9. Higgins C. B., Vatner S. F., Braunwald E. Parasympathetic control of the heart. Pharmacol Rev. 1973 Mar;25(1):119–155. [PubMed] [Google Scholar]
  10. Hooisma J., Slaaf D. W., Meeter E., Stevens W. F. The innervation of chick striated muscle fibers by the chick ciliary ganglion in tissue culture. Brain Res. 1975 Feb 21;85(1):79–85. doi: 10.1016/0006-8993(75)91009-4. [DOI] [PubMed] [Google Scholar]
  11. Kano M., Shimada Y. Innervation and acetylcholine sensitivity of skeletal muscle cells differentiated in vitro from chick embryo. J Cell Physiol. 1971 Oct;78(2):233–242. doi: 10.1002/jcp.1040780210. [DOI] [PubMed] [Google Scholar]
  12. LEVI-MONTALCINI R., MEYER H., HAMBURGER V. In vitro experiments on the effects of mouse sarcomas 180 and 37 on the spinal and sympathetic ganglia of the chick embryo. Cancer Res. 1954 Jan;14(1):49–57. [PubMed] [Google Scholar]
  13. Lee C. Y., Chang S. L., Kau S. T., Luh S. H. Chromatographic separation of the venom of Bungarus multicinctus and characterization of its components. J Chromatogr. 1972 Oct 5;72(1):71–82. doi: 10.1016/0021-9673(72)80009-8. [DOI] [PubMed] [Google Scholar]
  14. Mains R. E., Patterson P. H. Primary cultures of dissociated sympathetic neurons. II. Initial studies on catecholamine metabolism. J Cell Biol. 1973 Nov;59(2 Pt 1):346–360. doi: 10.1083/jcb.59.2.346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nastuk W. L., Manthey A. A., Gissen A. J. Activation and inactivation of postjunctional membrane receptors. Ann N Y Acad Sci. 1966 Jul 14;137(2):999–1014. doi: 10.1111/j.1749-6632.1966.tb50212.x. [DOI] [PubMed] [Google Scholar]
  16. Nelson P. G. Nerve and muscle cells in culture. Physiol Rev. 1975 Jan;55(1):1–61. doi: 10.1152/physrev.1975.55.1.1. [DOI] [PubMed] [Google Scholar]
  17. O'Lague P. H., MacLeish P. R., Nurse C. A., Claude P., Furshpan E. J., Potter D. D. Physiological and morphological studies on developing sympathetic neurons in dissociated cell culture. Cold Spring Harb Symp Quant Biol. 1976;40:399–407. doi: 10.1101/sqb.1976.040.01.038. [DOI] [PubMed] [Google Scholar]
  18. O'Lague P. H., Obata K., Claude P., Furshpan E. J., Potter D. D. Evidence for cholinergic synapses between dissociated rat sympathetic neurons in cell culture. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3602–3606. doi: 10.1073/pnas.71.9.3602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Patterson P. H., Chun L. L. The influence of non-neuronal cells on catecholamine and acetylcholine synthesis and accumulation in cultures of dissociated sympathetic neurons. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3607–3610. doi: 10.1073/pnas.71.9.3607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Purves R. D., Hill C. E., Chamley J. H., Mark G. E., Fry D. M., Burnstock G. Functional autonomic neuromuscular junctions in tissue culture. Pflugers Arch. 1974;350(1):1–7. doi: 10.1007/BF00586734. [DOI] [PubMed] [Google Scholar]
  21. Sastre A., Podleski T. R. Pharmacologic characterization of the Na+ ionophores in L6 myotubes. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1355–1359. doi: 10.1073/pnas.73.4.1355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shimada Y., Fischman D. A., Moscona A. A. The development of nerve-muscle junctions in monolayer cultures of embryonic spinal cord and skeletal muscle cells. J Cell Biol. 1969 Nov;43(2):382–387. doi: 10.1083/jcb.43.2.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. TRAUTWEIN W. Generation and conduction of impulses in the heart as affected by drugs. Pharmacol Rev. 1963 Jun;15:277–332. [PubMed] [Google Scholar]
  24. Veneroni G., Murray M. R. Formation de novo and development of neuromuscular junctions in vitro. J Embryol Exp Morphol. 1969 Apr;21(2):369–382. [PubMed] [Google Scholar]
  25. Yamamura H. I., Snyder S. H. Muscarinic cholinergic binding in rat brain. Proc Natl Acad Sci U S A. 1974 May;71(5):1725–1729. doi: 10.1073/pnas.71.5.1725. [DOI] [PMC free article] [PubMed] [Google Scholar]

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