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. 1977 Aug;74(8):3259–3263. doi: 10.1073/pnas.74.8.3259

Packaging recombinant DNA molecules into bacteriophage particles in vitro.

B Hohn, K Murray
PMCID: PMC431522  PMID: 333431

Abstract

Recombinant phage genomes made in reactions with purified enzymes may be recovered directly by packaging into phage heads in vitro. The process is efficient and nonselective and offers containment in initial stages of handling recombinant DNA. Ligase [poly(deoxyribonucleotide):poly-(deoxyribonucleotide) ligase (AMP-forming), EC 6.5.1.1] reaction products can recombine with endogenous phage DNA during packaging, but UV-irradiation eliminates the biological activity of the endogenous DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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