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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Aug;74(8):3451–3455. doi: 10.1073/pnas.74.8.3451

Tumor promoters inhibit spontaneous and induced differentiation of murine erythroleukemia cells in culture.

H Yamasaki, E Fibach, U Nudel, I B Weinstein, R A Rifkind, P A Marks
PMCID: PMC431603  PMID: 269404

Abstract

Addition of the potent tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), to murine erythroleukemia cell lines in suspension cultures inhibited both spontaneous differentiation and differentiation induced by hexamethylene bisacetamide (HMBA), dimethyl sulfoxide, or butyric acid. Inhibition was unrelated to cytotoxicity and was reversible. When several plant diterpenes were tested, there was a positive correlation between tumor-promoting activity and inhibition of differentiation. TPA inhibited HMBA-induced differentiation only if added prior to the time of commitment to differentiation, as assayed by scoring for differentiation after transfer of cells from HMBA to fresh medium without HMBA. TPA-mediated inhibition of differentiation was associated with a decrease in globin mRNA accumulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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