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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Aug;74(8):3471–3475. doi: 10.1073/pnas.74.8.3471

Biological properties of poliovirus encapsulated in lipid vesicles: antibody resistance and infectivity in virus-resistant cells.

T Wilson, D Papahadjopoulos, R Taber
PMCID: PMC431612  PMID: 198787

Abstract

We present evidence that poliovirus can be encapsulated in synthetic large phospholipid vesicles. The virus associated with the vesicles is found to be (i) resistant to antiserum against poliovirus and (ii) infectious for cells that are normally resistant to virus infection because of a membrane restriction. Our interpretation of these results is that the virus is entrapped in the interior aqueous space of the vesicles and that this vesicle-associated virus is introduced directly into the cytoplasm of the cells via fusion of the vesicles with the cellular plasma membrane, bypassing the surface receptor-mediated restriction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cohen C. M., Weissmann G., Hoffstein S., Awasthi Y. C., Srivastava S. K. Introduction of purified hexosaminidase A into Tay-Sachs leukocytes by means of immunoglobulin-coated liposomes. Biochemistry. 1976 Jan 27;15(2):452–460. doi: 10.1021/bi00647a034. [DOI] [PubMed] [Google Scholar]
  2. Enders J. F., Holloway A., Grogan E. A. Replication of poliovirus I in chick embryo and hamster cells exposed to sendai virus. Proc Natl Acad Sci U S A. 1967 Mar;57(3):637–644. doi: 10.1073/pnas.57.3.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gregoriadis G., Buckland R. A. Enzyme-containing liposomes alleviate a model for storage disease. Nature. 1973 Jul 20;244(5412):170–172. doi: 10.1038/244170a0. [DOI] [PubMed] [Google Scholar]
  4. Gregoriadis G. The carrier potential of liposomes in biology and medicine (first of two parts). N Engl J Med. 1976 Sep 23;295(13):704–710. doi: 10.1056/NEJM197609232951305. [DOI] [PubMed] [Google Scholar]
  5. HOLLAND J. J., McLAREN L. C., SYVERTON J. T. The mammalian cell-virus relationship. IV. Infection of naturally insusceptible cells with enterovirus ribonucleic acid. J Exp Med. 1959 Jul 1;110(1):65–80. doi: 10.1084/jem.110.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lonberg-Holm K., Gosser L. B., Shimshick E. J. Interaction of liposomes with subviral particles of poliovirus type 2 and rhinovirus type 2. J Virol. 1976 Aug;19(2):746–749. doi: 10.1128/jvi.19.2.746-749.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lonberg-Holm K., Whiteley N. M. Physical and metabolic requirements for early interaction of poliovirus and human rhinovirus with HeLa cells. J Virol. 1976 Sep;19(3):857–870. doi: 10.1128/jvi.19.3.857-870.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Magee W. E., Goff C. W., Schoknecht J., Smith M. D., Cherian K. The interaction of cationic liposomes containing entrapped horseradish peroxidase with cells in culture. J Cell Biol. 1974 Nov;63(2 Pt 1):492–504. doi: 10.1083/jcb.63.2.492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Oppermann H., Koch G. Kinetics of poliovirus replication in HeLa cells infected by isolated RNA. Biochem Biophys Res Commun. 1973 May 15;52(2):635–640. doi: 10.1016/0006-291x(73)90760-2. [DOI] [PubMed] [Google Scholar]
  10. Papahadjopoulos D., Miller N. Phospholipid model membranes. I. Structural characteristics of hydrated liquid crystals. Biochim Biophys Acta. 1967 Sep 9;135(4):624–638. doi: 10.1016/0005-2736(67)90094-6. [DOI] [PubMed] [Google Scholar]
  11. Papahadjopoulos D., Poste G., Mayhew E. Cellular uptake of cyclic AMP captured within phospholipid vesicles and effect on cell-growth behaviour. Biochim Biophys Acta. 1974 Sep 23;363(3):404–418. doi: 10.1016/0005-2736(74)90079-0. [DOI] [PubMed] [Google Scholar]
  12. Papahadjopoulos D., Vail W. J., Jacobson K., Poste G. Cochleate lipid cylinders: formation by fusion of unilamellar lipid vesicles. Biochim Biophys Acta. 1975 Jul 3;394(3):483–491. doi: 10.1016/0005-2736(75)90299-0. [DOI] [PubMed] [Google Scholar]
  13. Poste G., Papahadjopoulos D. Lipid vesicles as carriers for introducing materials into cultured cells: influence of vesicle lipid composition on mechanism(s) of vesicle incorporation into cells. Proc Natl Acad Sci U S A. 1976 May;73(5):1603–1607. doi: 10.1073/pnas.73.5.1603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tyrrell D. A., Heath T. D., Colley C. M., Ryman B. E. New aspects of liposomes. Biochim Biophys Acta. 1976 Dec 14;457(3-4):259–302. doi: 10.1016/0304-4157(76)90002-2. [DOI] [PubMed] [Google Scholar]
  15. Weinstein J. N., Yoshikami S., Henkart P., Blumenthal R., Hagins W. A. Liposome-cell interaction: transfer and intracellular release of a trapped fluorescent marker. Science. 1977 Feb 4;195(4277):489–492. doi: 10.1126/science.835007. [DOI] [PubMed] [Google Scholar]

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