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. 1977 Dec;74(12):5305–5309. doi: 10.1073/pnas.74.12.5305

Phage T4-modified RNA polymerase transcribes T4 late genes in vitro.

D Rabussay, E P Geiduschek
PMCID: PMC431695  PMID: 271954

Abstract

Initiation of T4 late RNA synthesis has been achieved in an in vitro system prepared from Escherichia coli cells infected with wild-type or maturation-defective mutant T4 phage. The system uses a cellophane membrane as a mechanical support for concentrated cell lysates and for added streptolydigin-resistant RNA polymerases. Transcriptional activity and selectivity of added RNA polymerases are tested while endogenous RNA polymerase activity is inhibited by streptolydigin. T4-modified RNA polymerase is required for substantial stimulation of T4 late RNA synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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