Abstract
The primary structure of the membranous segment of porcine liver microsomal cytochrome b5 has been determined. This polypeptide is at the COOH terminus of the cytochrome molecule and consists of 43 amino acids. It is essential for the insertion of the cytochrome into the endoplasmic reticular membrane. Automated sequence analysis of tryptic and cyanogen bromide/anhydrous heptafluorobutyric acid peptides provided data from which the following unique amino acid sequence was deduced: Ile-Ala-Lys-Pro-Ser-Glu-Thr-Leu-Ile-Thr-Thr-Val-Glu-Ser-Asn-Ser-Ser-Trp-Trp-Thr-Asn-Trp-Val-Ile-Pro-Ala-Ile-Ser-Ala-Leu-Val-Val-Ser-Leu-Met-Tyr-His-Phe-Tyr-Thr-Ser-Glu-Asn. A prediction of alpha-helices, beta-structures, and beta-turns basedon the sequence of this polypeptide is also presented.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chou P. Y., Fasman G. D. Prediction of protein conformation. Biochemistry. 1974 Jan 15;13(2):222–245. doi: 10.1021/bi00699a002. [DOI] [PubMed] [Google Scholar]
- Cohen B. S., Estabrook R. W. Microsomal electron transport reactions. 3. Cooperative interactions between reduced diphosphopyridine nucleotide and reduced triphosphopyridine nucleotide linked reactions. Arch Biochem Biophys. 1971 Mar;143(1):54–65. doi: 10.1016/0003-9861(71)90185-8. [DOI] [PubMed] [Google Scholar]
- Fasman G. D., Chou P. Y., Adler A. J. Prediction of the conformation of the histones. Biophys J. 1976 Oct;16(10):1201–1238. doi: 10.1016/S0006-3495(76)85768-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nóbrega F. G., Ozols J. Amino acid sequences of tryptic peptides of cytochromes b5 from microsomes of human, monkey, porcine, and chicken liver. J Biol Chem. 1971 Mar 25;246(6):1706–1717. [PubMed] [Google Scholar]
- Oshino N., Sato R. Stimulation by phenols of the reoxidation microsomal bound cytochrome b5 and its implication to fatty acid desaturation. J Biochem. 1971 Jan;69(1):169–180. doi: 10.1093/oxfordjournals.jbchem.a129445. [DOI] [PubMed] [Google Scholar]
- Ozols J. Amino acid sequence of rabbit liver microsomal cytochrome b5. J Biol Chem. 1970 Oct 10;245(19):4863–4874. [PubMed] [Google Scholar]
- Ozols J. Cytochrome b 5 from a normal human liver. Isolation and the partial amino acid sequence. J Biol Chem. 1972 Apr 10;247(7):2242–2245. [PubMed] [Google Scholar]
- Ozols J. Cytochrome b5 from microsomal membranes of equine, bovine, and porcine livers. Isolation and properties of preparations containing the membranous segment. Biochemistry. 1974 Jan 29;13(3):426–434. doi: 10.1021/bi00700a005. [DOI] [PubMed] [Google Scholar]
- Ozols J., Gerard C., Nobrega F. G. Proteolytic cleavage of horse liver cytochrome b5. Primary structure of the heme-containing moiety. J Biol Chem. 1976 Nov 10;251(21):6767–6774. [PubMed] [Google Scholar]
- Shimakata T., Mihara K., Sato R. Reconstitution of hepatic microsomal stearoyl-coenzyme A desaturase system from solubilized components. J Biochem. 1972 Nov;72(5):1163–1174. doi: 10.1093/oxfordjournals.jbchem.a130004. [DOI] [PubMed] [Google Scholar]
- Strittmatter P., Rogers M. J., Spatz L. The binding of cytochrome b 5 to liver microsomes. J Biol Chem. 1972 Nov 25;247(22):7188–7194. [PubMed] [Google Scholar]
- Strittmatter P., Spatz L., Corcoran D., Rogers M. J., Setlow B., Redline R. Purification and properties of rat liver microsomal stearyl coenzyme A desaturase. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4565–4569. doi: 10.1073/pnas.71.11.4565. [DOI] [PMC free article] [PubMed] [Google Scholar]