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. 1977 Sep;74(9):3869–3873. doi: 10.1073/pnas.74.9.3869

Low-molecular-weight peptide inhibits RNA synthesis in human leukemic and phytohemagglutinin-stimulated leukocytes and globin mRNA transcription in differentiating Friend cells.

D Amici, G B Rossi, L Cioé, G P Matarese, A Dolei, L Guglielmi, G L Gianfranceschi
PMCID: PMC431764  PMID: 269438

Abstract

The RNA synthesis of human leukemic leukocytes and phytohemagglutinin-stimulated lymphocytes is markedly reduced by administration of a low-molecular-weight nonhistone peptide factor from calf thymus. Treatment with the factor strongly inhibits hemoglobin production and globin mRNA transcription in dimethyl sulfoxide-stimulated Friend cells without appreciably modifying the rate of cell growth. Evidence for specificity of these effects is provided by the lack of action of the factor on both growth rate and RNA synthesis of a number of nondifferentiating cell lines from various animal species. After removal of the compound, both human lymphocytes and Friend cells can be stimulated by phytohemagglutinin and by dimethyl sulfoxide, respectively, ruling out any toxic effect.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CROSBY W. H., FURTH F. W. A modification of the benzidine method for measurement of hemoglobin in plasma and urine. Blood. 1956 Apr;11(4):380–383. [PubMed] [Google Scholar]
  2. Cline M. J., MacKenzie M. R. Synthesis of macroglobulins in vitro. Nature. 1967 Apr 29;214(5087):496–497. doi: 10.1038/214496a0. [DOI] [PubMed] [Google Scholar]
  3. Cooper H. L. Ribonucleic acid metabolism in lymphocytes stimulated by phytohemagglutinin. II. Rapidly synthesized ribonucleic acid and te production of ribosomal ribonucleic acid. J Biol Chem. 1968 Jan 10;243(1):34–43. [PubMed] [Google Scholar]
  4. Forget B. G., Marotta C. A., Weissman S. M., Verma I. M., McCaffrey R. P., Baltimore D. Nucleotide sequences of human globin messenger RNA. Ann N Y Acad Sci. 1974 Nov 29;241(0):290–309. doi: 10.1111/j.1749-6632.1974.tb21888.x. [DOI] [PubMed] [Google Scholar]
  5. Friend C., Scher W., Holland J. G., Sato T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378–382. doi: 10.1073/pnas.68.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gianfranceschi G. L., Amici D., Guglielmi L. Evidence for the presence in calf thymus of a peptidic factor controlling DNA transcription in vitro. Biochim Biophys Acta. 1975 Nov 18;414(1):9–19. doi: 10.1016/0005-2787(75)90121-5. [DOI] [PubMed] [Google Scholar]
  7. Gianfranceschi G. L., Amici D., Guglielmi L. Restriction of template capacity of rat liver chromatin by a non-histone peptide from calf thymus. Nature. 1976 Aug 12;262(5569):622–623. doi: 10.1038/262622a0. [DOI] [PubMed] [Google Scholar]
  8. Gianfranceschi G. L., Amici D., Guglielmi L. Stabilization of double-stranded DNA molecule by non-histone peptidic effector from calf thymus. Mol Biol Rep. 1976 Sep;3(1):55–64. doi: 10.1007/BF00357209. [DOI] [PubMed] [Google Scholar]
  9. Goldstein A. L., Banerjee S., White A. Preparation and properties of thymostatin, a new thymic inhibitor of DNA and RNA synthesis. Proc Natl Acad Sci U S A. 1967 Mar;57(3):821–828. doi: 10.1073/pnas.57.3.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Orkin S. H., Harosi F. I., Leder P. Differentiation in erythroleukemic cells and their somatic hybrids. Proc Natl Acad Sci U S A. 1975 Jan;72(1):98–102. doi: 10.1073/pnas.72.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pogo B. G., Allfrey V. G., Mirsky A. E. RNA synthesis and histone acetylation during the course of gene activation in lymphocytes. Proc Natl Acad Sci U S A. 1966 Apr;55(4):805–812. doi: 10.1073/pnas.55.4.805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Preisler H. S., Housman D., Scher W., Friend C. Effects of 5-bromo-2' -deoxyuridine on production of globin messenger RNA in dimethyl sulfoxide-stimulated Friend leukemia cells. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2956–2959. doi: 10.1073/pnas.70.10.2956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rossi G. B., Dolei A., Cioé L., Benedetto A., Matarese G. P., Belardelli F. Inhibition of transcription and translation of globin messenger RNA in dimethyl sulfoxide-stimulated Friend erythroleukemic cells treated with interferon. Proc Natl Acad Sci U S A. 1977 May;74(5):2036–2040. doi: 10.1073/pnas.74.5.2036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sawada H., Gilmore V. H., Saunders G. F. Transcription from chromatins of human lymphocytic leukemia cells and normal lymphocytes. Cancer Res. 1973 Feb;33(2):428–434. [PubMed] [Google Scholar]
  15. Szent-Györgyi A., Hegyeli A., McLaughlin J. A. CONSTITUENTS OF THE THYMUS GLAND AND THEIR RELATION TO GROWTH, FERTILITY, MUSCLE, AND CANCER. Proc Natl Acad Sci U S A. 1962 Aug;48(8):1439–1442. doi: 10.1073/pnas.48.8.1439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Trainin N. Thymic hormones and the immune response. Physiol Rev. 1974 Apr;54(2):272–315. doi: 10.1152/physrev.1974.54.2.272. [DOI] [PubMed] [Google Scholar]

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