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. 1977 Dec;74(12):5487–5491. doi: 10.1073/pnas.74.12.5487

Maize chloroplast DNA fragment encoding the large subunit of ribulosebisphosphate carboxylase

Donald M Coen *, John R Bedbrook †,‡, Lawrence Bogorad †,§, Alexander Rich *
PMCID: PMC431774  PMID: 16592473

Abstract

In vitro linked transcription-translation of chloroplast DNA has been used to show that the large subunit of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39] is encoded by Zea mays chloroplast DNA. A BamHI-generated chloroplast DNA sequence cloned in Escherichia coli is shown to direct the in vitro synthesis of this protein identified as large subunit by its size, serological properties, and limited proteolytic digestion products.

Keywords: molecular cloning, restriction fragments, linked transcription-translation

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Selected References

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