Abstract
A neutron small-angle scattering analysis has been done on partially deuterated 30S and 50S subunits of Escherichia coli ribosomes by the contrast variation method. The results indicate that the central regions of both particles are RNA-rich whereas their exteriors are protein-rich. The segregation of RNA and protein is much greater in the 50S subunit than in the 30S; the 30S subunit approaches a homogeneous mixture of RNA and protein. In both structures the most probable separation between the centers of mass of their protein and RNA distributions is small, although substantial variation of this parameter is possible within experimental error.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Moore P. B., Engelman D. M., Schoenborn B. P. A neutron scattering study of the distribution of protein and RNA in the 30 S ribosomal subunit of Escherichia coli. J Mol Biol. 1975 Jan 5;91(1):101–120. doi: 10.1016/0022-2836(75)90374-5. [DOI] [PubMed] [Google Scholar]
- Moore P. B., Engelman D. M., Schoenborn B. P. Asymmetry in the 50S ribosomal subunit of Escherichia coli. Proc Natl Acad Sci U S A. 1974 Jan;71(1):172–176. doi: 10.1073/pnas.71.1.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ortega J. P., Hill W. E. A molecular weight determination of the 16S ribosomal ribonucleic acid from Escherichia coli. Biochemistry. 1973 Aug 14;12(17):3241–3243. doi: 10.1021/bi00741a015. [DOI] [PubMed] [Google Scholar]
- SPAHR P. F. Amino acid composition of ribosomes from Escherichia coli. J Mol Biol. 1962 May;4:395–406. doi: 10.1016/s0022-2836(62)80020-5. [DOI] [PubMed] [Google Scholar]
- Schreier A. A., Baldwin R. L. Concentration-dependent hydrogen exchange kinetics of 3H-labeled S-peptide in ribonuclease S. J Mol Biol. 1976 Aug 15;105(3):409–426. doi: 10.1016/0022-2836(76)90101-7. [DOI] [PubMed] [Google Scholar]
- Stuhrmann H. B., Haas J., Ibel K., Wolf B., Koch M. H., Parfait R., Crichton R. R. New low resolution model for 50S subunit of Escherichia coli ribosomes. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2379–2383. doi: 10.1073/pnas.73.7.2379. [DOI] [PMC free article] [PubMed] [Google Scholar]