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. 1977 Sep;74(9):3965–3969. doi: 10.1073/pnas.74.9.3965

Excision of F plasmid sequences by recombination at directly repeated insertion sequence 2 elements: involvement of recA.

R C Deonier, L Mirels
PMCID: PMC431803  PMID: 333452

Abstract

The DNA of the F plasmid is joined to bacterial DNA sequences in the F' ORF203 by directly repeated insertion sequence 2 (IS2) elements. The rate of excision of the F plasmid form this F' (presumably by recombination at the directly repeated IS2s) has been estimated in both recA+ and recA- strains. Normal F is produced in the recA+ strain, but is not detected in recA-. The autonomous plasmids produced in the recA- background were F's having deletions. F excision in this particular recA+ case is specific in the sense that the directly repeated IS2s appear to be more active in recombination than similarly disposed IS3 direct repetitions in this F'.

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Selected References

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