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. 1977 Sep;74(9):3965–3969. doi: 10.1073/pnas.74.9.3965

Excision of F plasmid sequences by recombination at directly repeated insertion sequence 2 elements: involvement of recA.

R C Deonier, L Mirels
PMCID: PMC431803  PMID: 333452

Abstract

The DNA of the F plasmid is joined to bacterial DNA sequences in the F' ORF203 by directly repeated insertion sequence 2 (IS2) elements. The rate of excision of the F plasmid form this F' (presumably by recombination at the directly repeated IS2s) has been estimated in both recA+ and recA- strains. Normal F is produced in the recA+ strain, but is not detected in recA-. The autonomous plasmids produced in the recA- background were F's having deletions. F excision in this particular recA+ case is specific in the sense that the directly repeated IS2s appear to be more active in recombination than similarly disposed IS3 direct repetitions in this F'.

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Selected References

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  1. Berg C. M., Curtiss R., 3rd Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics. 1967 Jul;56(3):503–525. doi: 10.1093/genetics/56.3.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berg D. E., Gallant J. A. Tests of reciprocality in crossingover in partially diploid F strains of Escherichia coli. Genetics. 1971 Aug;68(4):457–472. doi: 10.1093/genetics/68.4.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Curtiss R., 3rd Genetic manipulation of microorganisms: potential benefits and biohazards. Annu Rev Microbiol. 1976;30:507–533. doi: 10.1146/annurev.mi.30.100176.002451. [DOI] [PubMed] [Google Scholar]
  5. Deonier R. C., Davidson N. The sequence organization of the integrated F plasmid in two Hfr strains of Escherichia coli. J Mol Biol. 1976 Nov 5;107(3):207–222. doi: 10.1016/s0022-2836(76)80002-2. [DOI] [PubMed] [Google Scholar]
  6. Deonier R. C., Oh G. R., Hu M. Further mapping of IS2 and IS3 in the lac-purE region of the Escherichia coli K-12 genome: structure of the F-prime ORF203. J Bacteriol. 1977 Feb;129(2):1129–1140. doi: 10.1128/jb.129.2.1129-1140.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Helling R. B., Goodman H. M., Boyer H. W. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974 Nov;14(5):1235–1244. doi: 10.1128/jvi.14.5.1235-1244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hsu M. T., Davidson N. Structure of inserted bacteriophage Mu-1 DNA and physical mapping of bacterial genes by Mu-1 DNA insertion. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2823–2827. doi: 10.1073/pnas.69.10.2823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hu S., Ohtsubo E., Davidson N. Electron microscopic heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F13 and related F-primes. J Bacteriol. 1975 May;122(2):749–763. doi: 10.1128/jb.122.2.749-763.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hu S., Ptashne K., Cohen S. N., Davidson N. alphabeta sequence of F is IS31. J Bacteriol. 1975 Aug;123(2):687–692. doi: 10.1128/jb.123.2.687-692.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jaskunas S. R., Lindahl L., Nomura M. Isolation of polar insertion mutants and the direction of transcription of ribosomal protein genes in E. coli. Nature. 1975 Jul 17;256(5514):183–187. doi: 10.1038/256183a0. [DOI] [PubMed] [Google Scholar]
  12. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  13. Lieb M. Forward and Reverse Mutation in a Histidine-Requiring Strain of Escherichia Coli. Genetics. 1951 Sep;36(5):460–477. doi: 10.1093/genetics/36.5.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Luria S. E., Delbrück M. Mutations of Bacteria from Virus Sensitivity to Virus Resistance. Genetics. 1943 Nov;28(6):491–511. doi: 10.1093/genetics/28.6.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Polisky B., Greene P., Garfin D. E., McCarthy B. J., Goodman H. M., Boyer H. W. Specificity of substrate recognition by the EcoRI restriction endonuclease. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3310–3314. doi: 10.1073/pnas.72.9.3310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Saedler H., Heiss B. Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K-12. Mol Gen Genet. 1973 May 9;122(3):267–277. doi: 10.1007/BF00278602. [DOI] [PubMed] [Google Scholar]
  18. Sharp P. A., Hsu M. T., Otsubo E., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol. 1972 Nov 14;71(2):471–497. doi: 10.1016/0022-2836(72)90363-4. [DOI] [PubMed] [Google Scholar]
  19. Thompson R., Hughes S. G., Broda P. Plasmid identification using specific endonucleases. Mol Gen Genet. 1974;133(2):141–149. doi: 10.1007/BF00264835. [DOI] [PubMed] [Google Scholar]

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