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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Dec;74(12):5551–5553. doi: 10.1073/pnas.74.12.5551

31P nuclear magnetic resonance measurements of ATPase kinetics in aerobic Escherichia coli cells.

T R Brown, K Ugurbil, R G Shulman
PMCID: PMC431804  PMID: 146199

Abstract

We have measured the in vivo unidirectional rates between the terminal phosphate of ATP and intracellular inorganic phosphate (PiIN) in aerobic suspensions of Escherichia coli cells using 31P nuclear magnetic resonance saturation transfer techniques. Typically, the measurements consisted of saturating the ATPgamma resonance and observing a 20 +/- 5% reduction in the intensity of the PiIN resonance. No saturation transfer was observed after incubation with 1 mM dicyclohexylcarbodiimide, an ATPase inhibitor. From the measured decrease in intensity of PiIN coming from saturation transfer, the apparent unimolecular rate constant of PiIN to ATP was calculated to be 0.6 +/- 0.15 sec-1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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