Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Sep;74(9):3970–3974. doi: 10.1073/pnas.74.9.3970

Specialized transducing phage for the initiation factor 3 gene in Escherichia coli.

M Springer, M Graffe, H Hennecke
PMCID: PMC431805  PMID: 333453

Abstract

A previously isolated thermosensitive mutant [Springer, M., Graffe, M. & Grunberg-Manago, M. (6977) Mol. Gen. Genet. 151, 17-26] exhibits two defects in vitro, one in the initiation factor IF3 and the other in the L-phenylalanine: tRNA-Phe ligase (EC 6.1.1.20). Specialized lambda transducing phages that transduced this mutant to thermoresistance were selected. In vitro studies showed that the transductants had a normal IF3 activity. One of these transducing phages was shown to code for a protein synthesized under the control of Escherichia coli promoters, which has the same molecular weight as IF3. This protein crossreacts specifically with IF3 antisera and comigrates with pure IF3 in a two-dimensional gel system.

Full text

PDF
3970

Images in this article

3972Image
on p.3972
3973Image
on p.3973
3973Image
on p.3973

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Böck A., Ruffler D., Piepersberg W., Wittmann H. G. Genetic analysis of an alteration of ribosomal protein S20 in revertants of an alanyl-tRNA-synthetase mutant of Escherichia coli. Mol Gen Genet. 1974;134(4):325–332. doi: 10.1007/BF00337467. [DOI] [PubMed] [Google Scholar]
  3. Cameron J. R., Panasenko S. M., Lehman I. R., Davis R. W. In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3416–3420. doi: 10.1073/pnas.72.9.3416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dondon J., Godefroy-Colburn T., Graffe M., Grunberg-Manago M. IF-3 requirements for initiation complex formation with synthetic messengers in E. coli system. FEBS Lett. 1974 Sep 1;45(1):82–87. doi: 10.1016/0014-5793(74)80816-1. [DOI] [PubMed] [Google Scholar]
  5. Friesen J. D., Parker J., Watson R. J., Bendiak D., Reeh S. V., Pedersen S., Fiil N. P. A transducing bacteriophage lambda carrying the structural gene for elongation factor Ts. Mol Gen Genet. 1976 Oct 18;148(1):93–98. doi: 10.1007/BF00268549. [DOI] [PubMed] [Google Scholar]
  6. Hennecke H., Springer M., Böck A. A specialized transducing lambda phage carrying the Escherichia coli genes for phenylalanyl-tRNA synthetase. Mol Gen Genet. 1977 Apr 29;152(3):205–210. doi: 10.1007/BF00268819. [DOI] [PubMed] [Google Scholar]
  7. JACOB F., CAMPBELL A. Sur le système de répression assurant l'immunité chez les bactéries lysogenes. C R Hebd Seances Acad Sci. 1959 Jun 1;248(22):3219–3221. [PubMed] [Google Scholar]
  8. Jaskunas S. R., Lindahl L., Nomura M. Specialized transducing phages for ribosomal protein genes of Escherichia coli. Proc Natl Acad Sci U S A. 1975 Jan;72(1):6–10. doi: 10.1073/pnas.72.1.6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  11. Lee-Huang S., Ochoa S. Messenger discriminating species of initiation factor F3. Nat New Biol. 1971 Dec 22;234(51):236–239. doi: 10.1038/newbio234236a0. [DOI] [PubMed] [Google Scholar]
  12. Lund E., Dahlberg J. E., Lindahl L., Jaskunas S. R., Dennis P. P., Nomura M. Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. coli. Cell. 1976 Feb;7(2):165–177. doi: 10.1016/0092-8674(76)90016-7. [DOI] [PubMed] [Google Scholar]
  13. Mets L. J., Bogorad L. Two-dimensional polyacrylamide gel electrophoresis: an improved method for ribosomal proteins. Anal Biochem. 1974 Jan;57(1):200–210. doi: 10.1016/0003-2697(74)90065-7. [DOI] [PubMed] [Google Scholar]
  14. Perricaudet M., Tiollais P. Defective bacteriophage lambda chromosome, potential vector for DNA fragments obtained after cleavage by Bacillus amyloliquefaciens endonuclease (BamI). FEBS Lett. 1975 Aug 1;56(1):7–11. doi: 10.1016/0014-5793(75)80099-8. [DOI] [PubMed] [Google Scholar]
  15. Ptashne M. ISOLATION OF THE lambda PHAGE REPRESSOR. Proc Natl Acad Sci U S A. 1967 Feb;57(2):306–313. doi: 10.1073/pnas.57.2.306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rambach A., Tiollais P. Bacteriophage lambda having EcoRI endonuclease sites only in the nonessential region of the genome. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3927–3930. doi: 10.1073/pnas.71.10.3927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Revel M., Aviv(Greenshpan) H., Groner Y., Pollack Y. Fractionation of translation initiation factor B (F3) into cistron-specific species. FEBS Lett. 1970 Aug 17;9(4):213–217. doi: 10.1016/0014-5793(70)80358-1. [DOI] [PubMed] [Google Scholar]
  18. Schiff N., Miller M. J., Wahba A. J. Purification and properties of chain initiation factor 3 from T4-infected and uninfected Escherichia coli MRE 600. Stimulation of translation of synthetic and natural messengers. J Biol Chem. 1974 Jun 25;249(12):3797–3802. [PubMed] [Google Scholar]
  19. Schrenk W. J., Weisberg R. A. A simple method for making new transducing lines of coliphage lambda. Mol Gen Genet. 1975;137(2):101–107. doi: 10.1007/BF00341676. [DOI] [PubMed] [Google Scholar]
  20. Springer M., Graffe M., Grunberg-Manago M. Characterization of an E. coli mutant with a thermolabile initiation factor IF3 activity. Mol Gen Genet. 1977 Feb 28;151(1):17–26. doi: 10.1007/BF00446908. [DOI] [PubMed] [Google Scholar]
  21. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
  22. Watson R. J., Parker J., Fiil N. P., Flaks J. G., Friesen J. D. New chromosomal location for structural genes of ribosomal proteins. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2765–2769. doi: 10.1073/pnas.72.7.2765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yoshida M., Rudland P. S. Ribosomal binding of bacteriophage RNA with different components of initiation factor F3. J Mol Biol. 1972 Jul 28;68(3):465–481. doi: 10.1016/0022-2836(72)90100-3. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES