Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Sep;74(9):4012–4016. doi: 10.1073/pnas.74.9.4012

Expression of Ia-like antigen molecules on human granulocytes during early phases of differentiation

R J Winchester *, G D Ross †, C I Jarowski †, C Y Wang *, J Halper *, H E Broxmeyer ‡
PMCID: PMC431823  PMID: 71738

Abstract

Human B lymphocyte antigens analogous to the murine Ia determinants were found on myeloblasts and promyelocytes but not on more mature granulocytes. This was apparent by fluorescent staining with both human alloantisera and rabbit antisera to the isolated Ia-like proteins. The cells of patients with chronic myelocytic leukemia showed this difference especially clearly. Separation of the myeloblasts and promyelocytes by multistep density gradient fractionation produced a marked enrichment of the positive cells. The remaining cells from higher density fractions were more-mature neutrophils that were essentially negative. In acute myeloid leukemia, in which myeloid cells early in differentiation predominate, the vast majority of cells were strongly positive. Similar results were obtained with normal bone marrow cells. Here also, only the early forms of the myeloid series separated by gradient centrifugation had Ia antigens. Evidence was also obtained for the presence of Ia determinants on cells with the appearance of early erythroid precursors. Support for the presence of the Ia determinants on granulocyte-macrophage committed stem cells was provided by the inhibition of granulocyte colony formation in agar cultures following preincubation of normal bone marrow with antiserum and complement.

Cross absorptions with purified preparations of immature cells provided evidence for the close similarity of the antigenic determinants on both myeloblasts and B cells. A 28,000-37,000-dalton bimolecular complex obtained from myeloblast membranes contained the Ia determinants and was similar to that obtained from peripheral blood B cell membranes.

Keywords: normal bone marrow, leukemias, committed stem cells, B lymphocytes

Full text

PDF
4012

Images in this article

4015Image
on p.4015

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Billing R., Rafizadeh B., Drew I., Hartman G., Gale R., Terasaki P. Human B-lymphocyte antigens expressed by lymphocytic and myelocytic leukemia cells. I. Detection by rabbit antisera. J Exp Med. 1976 Jul 1;144(1):167–178. doi: 10.1084/jem.144.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Billing R., Ting A., Terasaki P. I. Human B-lymphocyte antigens expressed by lymphocytic and myelocytic leukemia cells. II. Detection by human anti-B-cell alloantisera. J Natl Cancer Inst. 1977 Feb;58(2):199–203. doi: 10.1093/jnci/58.2.199. [DOI] [PubMed] [Google Scholar]
  3. Broxmeyer H. E., Moore M. A., Ralph P. Cell-free granulocyte colony inhibiting activity derived from human polymorphonuclear neutrophils. Exp Hematol. 1977 Mar;5(2):87–102. [PubMed] [Google Scholar]
  4. Cullen S. E., Schwartz B. D. An improved method for isolation of H-2 and Ia alloantigens with immunoprecipitation induced by protein A-bearing staphylococci. J Immunol. 1976 Jul;117(1):136–142. [PubMed] [Google Scholar]
  5. Fu S. M., Winchester R. J., Kunkel H. G. The occurrence of the HL-B alloantigens on the cells of unclassified acute lymphoblastic leukemias. J Exp Med. 1975 Nov 1;142(5):1334–1338. doi: 10.1084/jem.142.5.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Greaves M. F., Brown G., Rapson N. T., Lister T. A. Antisera to acute lymphoblastic leukemia cells. Clin Immunol Immunopathol. 1975 May;4(1):67–84. doi: 10.1016/0090-1229(75)90041-0. [DOI] [PubMed] [Google Scholar]
  7. Humphreys R. E., McCune J. M., Chess L., Herrman H. C., Malenka D. J., Mann D. L., Parham P., Schlossman S. F., Strominger J. L. Isolation and immunologic characterization of a human. B-lymphocyte-specific, cell surface antigen. J Exp Med. 1976 Jul 1;144(1):98–112. doi: 10.1084/jem.144.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Janossy G., Roberts M., Greaves M. F. Target cell in chronic myeloid leukaemia and its relationship to acute lymphoid leukaemia. Lancet. 1976 Nov 13;2(7994):1058–1061. doi: 10.1016/s0140-6736(76)90970-3. [DOI] [PubMed] [Google Scholar]
  9. Jerry L. M., Lewis M. G., Rowden G., Sullivan A. K., Pitzele R., Law T. Fetal antigens in nonneoplastic conditions. Cancer Res. 1976 Sep;36(9 Pt 2):3446–3452. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Marchalonis J. J., Cone R. E., Santer V. Enzymic iodination. A probe for accessible surface proteins of normal and neoplastic lymphocytes. Biochem J. 1971 Oct;124(5):921–927. doi: 10.1042/bj1240921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pike B. L., Robinson W. A. Human bone marrow colony growth in agar-gel. J Cell Physiol. 1970 Aug;76(1):77–84. doi: 10.1002/jcp.1040760111. [DOI] [PubMed] [Google Scholar]
  13. Schlossman S. F., Chess L., Humphreys R. E., Strominger J. L. Distribution of Ia-like molecules on the surface of normal and leukemic human cells. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1288–1292. doi: 10.1073/pnas.73.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Winchester R. J., Wang C. Y., Halper J., Hoffman T. Studies with B-cell allo- and hetero-antisera: parallel reactivity and special properties. Scand J Immunol. 1976;5(6-7):745–757. doi: 10.1111/j.1365-3083.1976.tb03024.x. [DOI] [PubMed] [Google Scholar]
  15. Winchester R. J., Wernet P., Kunkel H. G., Dupont B., Jersild C., Fu S. M. Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes. J Exp Med. 1975 Apr 1;141(4):924–929. [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES