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. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Cell. 2014 Jan 30;156(4):649–662. doi: 10.1016/j.cell.2014.01.020

Figure 5. Ultrafast cycling cells emerge from fibroblasts after Yamanaka factor expression and harbor the majority of reprogramming activity.

Figure 5

Factor transduced MEFs were treated with Dox for 4 days, labeled with CFSE and allowed for dye-dilution for 48 hours in the presence of Dox. (A) The levels of CSFE in non-labeled MEFs (negative), right after labeling (post-label), or following 48 hours of dilution (post-dilution) are shown. The gates for fast, medium (med) and slow cycling cells are shown. Note that the fluorescence intensity difference between fast and medium populations indicates >4 division (div.) differences. (B) Reprogramming efficiency of cells sorted on CFSE. Oct4:GFP+ colonies were scored on day 20 from initial Dox induction (n=3 per condition, error bars indicate standard deviation). (C) Representative images of the reprogramming cultures from cells of different cycling speed. Phase and Oct4:GFP images were captured at 10x magnification and alkaline phosphatase (AP) stained dishes were from whole 60mm plates. See also Figure S6.