Abstract
The excitation of hemoproteins containing either Fe2+ or Fe3+ with a picosecond light pulse resulted in the bleaching and recovery of the iron absorbance bands within 6 psec. A new absorbance band also was observed whose formation and decay rate of less than 6 psec suggests that it is probably due to S1→Sn absorbance. The Soret band relaxation time is deduced to be ∼0.5 psec whereas the free base hemoprotein does not show any of the above fast-decay characteristics. Therefore, there is a strong influence of the metal on the energy dissipation process.
Keywords: picosecond spectroscopy, cytochromes, porphyrins
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brunner H. Resonance Raman scattering on the haem group of cytochrome c. Biochem Biophys Res Commun. 1973 Apr 16;51(4):888–894. doi: 10.1016/0006-291x(73)90010-7. [DOI] [PubMed] [Google Scholar]
- Straub K. D. A solid state theory of oxidative phosphorylation. J Theor Biol. 1974 Apr;44(2):191–206. doi: 10.1016/0022-5193(74)90156-8. [DOI] [PubMed] [Google Scholar]
- Strekas T. C., Spiro T. G. Hemoglobin: resonance Raman spectra. Biochim Biophys Acta. 1972 May 18;263(3):830–833. doi: 10.1016/0005-2795(72)90072-4. [DOI] [PubMed] [Google Scholar]
- TEALE F. W. The ultraviolet fluorescence of proteins in neutral solution. Biochem J. 1960 Aug;76:381–388. doi: 10.1042/bj0760381. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TEALE F. W., WEBER G. Ultraviolet fluorescence of the aromatic amino acids. Biochem J. 1957 Mar;65(3):476–482. doi: 10.1042/bj0650476. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEBER G. Enumeration of components in complex systems by fluorescence spectrophotometry. Nature. 1961 Apr 1;190:27–29. doi: 10.1038/190027a0. [DOI] [PubMed] [Google Scholar]
- Yammoto T., Palmer G. The valence and spin state of iron in oxyhemoglobin as inferred from resonance Raman spectroscopy. J Biol Chem. 1973 Jul 25;248(14):5211–5213. [PubMed] [Google Scholar]