Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Oct;74(10):4160–4162. doi: 10.1073/pnas.74.10.4160

Action of nucleotide phosphotransferase of Escherichia coli on nicotinamide riboside and nicotinamide mononucleotide.

E F Brunngraber, E Chargaff
PMCID: PMC431897  PMID: 144913

Abstract

The action of the nucleotide phosphotransferase of Escherichia coli on nicotinamide riboside and on its 5'-phosphate results in the addition of one phosphate moiety to each of the substrates. Although the proof is not conclusive, it is likely that the phosphate group is transferred to the 3'-hydroxyl of the ribose. This is in contrast to the behavior of the enzyme toward NAD in which only the adenylic acid portion is phosphorylated enzymically.

Full text

PDF
4160

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brunngraber E. F., Chargaff E. A nucleotide phosphotransferase from Escherichia coli. Purification and properties. Biochemistry. 1973 Jul 31;12(16):3005–3012. doi: 10.1021/bi00740a009. [DOI] [PubMed] [Google Scholar]
  2. Brunngraber E. F., Chargaff E. Nicotinamide adenine dinucleotide as substrate of the nucleotide phosphotransferase from Escherichia coli. Biochemistry. 1973 Jul 31;12(16):3012–3016. doi: 10.1021/bi00740a010. [DOI] [PubMed] [Google Scholar]
  3. Brunngraber E. F., Chargaff E. Nucleotide phosphotransferase of Escherichia coli: purification by affinity chromatography. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3226–3229. doi: 10.1073/pnas.74.8.3226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brunngraber E. F., Chargaff E. Purification and properties of a nucleoside phosphotransferase from carrot. J Biol Chem. 1967 Oct 25;242(20):4834–4840. [PubMed] [Google Scholar]
  5. Brunngraber E. F., Chargaff E. Transferase from Escherichia coli effecting low-energy phosphate transfer to nucleosides and nucleotides. Proc Natl Acad Sci U S A. 1970 Sep;67(1):107–112. doi: 10.1073/pnas.67.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. COLOWICK S. P., KAPLAN N. O., CIOTTI M. M. The reaction of pyridine nucleotide with cyanide and its analytical use. J Biol Chem. 1951 Aug;191(2):447–459. [PubMed] [Google Scholar]
  7. KODKICEK E., REDDI K. K. Paper chromatography of nicotinic acid derivatives. Nature. 1951 Sep 15;168(4272):475–477. doi: 10.1038/168475b0. [DOI] [PubMed] [Google Scholar]
  8. KORNBERG A., PRICER W. E., Jr On the structure of triphosphopyridine nucleotide. J Biol Chem. 1950 Oct;186(2):557–567. [PubMed] [Google Scholar]
  9. SHUSTER L., KAPLAN N. O. A specific b nucleotidase. J Biol Chem. 1953 Apr;201(2):535–546. [PubMed] [Google Scholar]
  10. SULKOWSKI E., BJORK W., LASKOWSKI M., Sr A specific and nonspecific alkaline monophosphatase in the venom of Bothrops atrox and their occurrence in the purified venom phosphodiesterase. J Biol Chem. 1963 Jul;238:2477–2486. [PubMed] [Google Scholar]
  11. TUNIS M., CHARGAFF E. Studies on the nucleoside phosphotransferase of carrot. III. On the synthetic ability of the transfer enzyme. Biochim Biophys Acta. 1960 May 20;40:206–210. doi: 10.1016/0006-3002(60)91343-3. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES