Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Oct;74(10):4243–4247. doi: 10.1073/pnas.74.10.4243

Chemical basis for brain-specific serine transfer RNAs.

H Rogg, P Müller, G Keith, M Staehelin
PMCID: PMC431915  PMID: 270667

Abstract

Serine tRNA from rat brain can be resolved into six isoaccepting species. Three of these species show the same chromatographic behavior as the seryl tRNAs from other rat organs, whereas the remaining species appear to be specific for brain. The isoacceptor tRNAs were purified to homogeneity by chromatography on benzoylated DEAE-cellulose followed by reversed-phase chromatography. We found that the additional species of serine tRNA in brain differ from their counterparts derived from other rat organs by a lack of a specific guanosine ribose-methylation in the dihydrouridine loop. In addition, when total liver tRNA was compared with total brain tRNA, the same degree of undermethylation with respect to 2'-O-methylguanosine was found as a general phenomenon.

Full text

PDF
4243

Images in this article

4246Image
on p.4246

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chia L. L., Randerath K., Randerath E. Base analysis of ribopolynucleotides by tritium incorporation following analytical polyacrylamide gel electrophoresis. Anal Biochem. 1973 Sep;55(1):102–113. doi: 10.1016/0003-2697(73)90295-9. [DOI] [PubMed] [Google Scholar]
  2. Frazer J. M., Yang W. K. Isoaccepting transfer ribonucleic acids in liver and brain of young and old BC3F 1 mice. Arch Biochem Biophys. 1972 Dec;153(2):610–618. doi: 10.1016/0003-9861(72)90380-3. [DOI] [PubMed] [Google Scholar]
  3. Hatfield D., Portugal F. H., Caicuts M. Transfer RNA specificity in mammalian tissues and codon responses of seryl transfer RNA. Cancer Res. 1971 May;31(5):697–700. [PubMed] [Google Scholar]
  4. Hatfield D., Portugal F. H. Seryl-tRNA in mammalian tissues: chromatographic differences in brain and liver and a specific response to the codon, UGA. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1200–1206. doi: 10.1073/pnas.67.3.1200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kim S. H., Sussman J. L., Suddath F. L., Quigley G. J., McPherson A., Wang A. H., Seeman N. C., RICH A. The general structure of transfer RNA molecules. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4970–4974. doi: 10.1073/pnas.71.12.4970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kothari R. M., Taylor M. W. RNA fractionation on reversed-phase columns. J Chromatogr. 1973 Nov 21;86(2):289–324. [PubMed] [Google Scholar]
  7. Littauer U. Z., Inouye H. Regulation of tRNA. Annu Rev Biochem. 1973;42:439–470. doi: 10.1146/annurev.bi.42.070173.002255. [DOI] [PubMed] [Google Scholar]
  8. Morisawa S., Chargaff E. On the bias of the distribution of the 2'-O-methylribonucleotide constituents of yeast transfer RNA. Biochim Biophys Acta. 1968 Dec 17;169(2):285–296. doi: 10.1016/0005-2787(68)90037-3. [DOI] [PubMed] [Google Scholar]
  9. Murthy M. R., Roux H., Thenot J. P. Isoacceptor tRNAs for glutamate, glutamine, aspartate and asparagine in calf brain. J Neurochem. 1974 Jan;22(1):19–22. doi: 10.1111/j.1471-4159.1974.tb12174.x. [DOI] [PubMed] [Google Scholar]
  10. Müller P., Wehrli W., Staehelin M. Isolation and characterization of serine transfer ribonucleic acids from rat liver. Biochemistry. 1971 May 11;10(10):1885–1890. doi: 10.1021/bi00786a024. [DOI] [PubMed] [Google Scholar]
  11. Pike L. M., Rottman F. The determination of 2'-O-methylnucleosides in RNA. Anal Biochem. 1974 Oct;61(2):367–378. doi: 10.1016/0003-2697(74)90404-7. [DOI] [PubMed] [Google Scholar]
  12. Quigley G. J., Seeman N. C., Wang A. H., Suddath F. L., Rich A. Yeast phenylalanine transfer RNA: atomic coordinates and torsion angles. Nucleic Acids Res. 1975 Dec;2(12):2329–2341. doi: 10.1093/nar/2.12.2329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Randerath K., MacKinnon S. K., Randerath E. An investigation of the minor base composition of transfer RNA in normal human brain and malignant brain tumors. FEBS Lett. 1971 Jun 2;15(1):81–84. doi: 10.1016/0014-5793(71)80084-4. [DOI] [PubMed] [Google Scholar]
  14. Richardson C. C. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. Proc Natl Acad Sci U S A. 1965 Jul;54(1):158–165. doi: 10.1073/pnas.54.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Richter D., Erdmann V. A., Sprinzl M. Specific recognition of GTpsiC loop (loop IV) of tRNA by 50S ribosomal subunits from E. coli. Nat New Biol. 1973 Dec 5;246(153):132–135. doi: 10.1038/newbio246132a0. [DOI] [PubMed] [Google Scholar]
  16. Rogg H., Brambilla R., Keith G., Staehelin M. An improved method for the separation and quantitation of the modified nucleosides of transfer RNA. Nucleic Acids Res. 1976 Jan;3(1):285–295. doi: 10.1093/nar/3.1.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rogg H., Staehelin M. Nucleotide sequences of rat liver serine-tRNA. 2. The products of digestion with ribonuclease T. Eur J Biochem. 1971 Jul 29;21(2):243–248. doi: 10.1111/j.1432-1033.1971.tb01462.x. [DOI] [PubMed] [Google Scholar]
  18. Rogg H., Wehrli W., Staehelin M. Isolation of mammalian transfer RNA. Biochim Biophys Acta. 1969 Nov 19;195(1):13–15. doi: 10.1016/0005-2787(69)90597-8. [DOI] [PubMed] [Google Scholar]
  19. Schwarz U., Lührmann R., Gassen H. G. On the mRNA induced conformational change of AA-tRNA exposing the T-pse-C-G sequence for binding to the 50S ribosomal subunit. Biochem Biophys Res Commun. 1974 Feb 4;56(3):807–814. doi: 10.1016/0006-291x(74)90677-9. [DOI] [PubMed] [Google Scholar]
  20. Simsek M., Ziegenmeyer J., Heckman J., Rajbhandary U. L. Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1041–1045. doi: 10.1073/pnas.70.4.1041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Staehelin M., Rogg H., Baguley B. C., Ginsberg T., Wehrli W. Structure of a mammalian serine tRNA. Nature. 1968 Sep 28;219(5161):1363–1365. doi: 10.1038/2191363a0. [DOI] [PubMed] [Google Scholar]
  22. Sueoka N., Kano-Sueoka T. Transfer RNA and cell differentiation. Prog Nucleic Acid Res Mol Biol. 1970;10:23–55. doi: 10.1016/s0079-6603(08)60560-7. [DOI] [PubMed] [Google Scholar]
  23. Watanabe K., Shinma M., Oshima T., Nishimura S. Heat-induced stability of tRNA from an extreme thermophile, Thermus thermophilus. Biochem Biophys Res Commun. 1976 Oct 4;72(3):1137–1144. doi: 10.1016/s0006-291x(76)80250-1. [DOI] [PubMed] [Google Scholar]
  24. Wehrli W., Staehelin M. Fractionation of the nonpolar transfer ribonucleic acids from rat liver, yeast, and Escherichia coli by partition chromatography. Biochemistry. 1971 May 11;10(10):1878–1885. doi: 10.1021/bi00786a023. [DOI] [PubMed] [Google Scholar]
  25. Weiss J. F., Pearson R. L., Kelmers A. D. Two additional reversed-phase chromatographic systems for the separation of transfer ribonucleic acids and their application to the preparation of two formylmethionine and a valine transfer ribonucleic acid from Escherichia coli B. Biochemistry. 1968 Oct;7(10):3479–3487. doi: 10.1021/bi00850a024. [DOI] [PubMed] [Google Scholar]
  26. Yang W. K., Novelli G. D. Multiple isoaccepting transfer RNA's in a muouse plasma cell tumor. Proc Natl Acad Sci U S A. 1968 Jan;59(1):208–215. doi: 10.1073/pnas.59.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES