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. 1977 Oct;74(10):4370–4374. doi: 10.1073/pnas.74.10.4370

Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.

B M Paterson, B E Roberts, E L Kuff
PMCID: PMC431943  PMID: 270678

Abstract

We present a simple method for directly correlating structural gene sequences in DNA with their corresponding mRNAs. This is based upon the fact that mRNA hybridized with its complementary DNA will not direct the cell-free synthesis of a complete polypeptide. Full translational activity of the mRNA is recovered upon the heat melting of the hybrid. Utilizing the rabbit beta globin clone PbetaG1, we demonstrate the application of hybrid-arrested translation for the identification of structural gene sequences within recombinant DNA molecules. In addition, the method is used to locate and order precisely several adenovirus 2 polypeptides within the viral genome.

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Selected References

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